...
首页> 外文期刊>Journal of dairy research >Estimation of inbreeding in cattle using RAPD markers
【24h】

Estimation of inbreeding in cattle using RAPD markers

机译:使用RAPD标记估算牛的近交

获取原文
获取原文并翻译 | 示例
           

摘要

Inbreeding indicates the degree of homozygosity at a locus within a population. Normally inbreeding is estimated in terms of a coefficient calculated from the pedigree of an individual. If no history is available, however, there is no way to estimate the inbreeding coefficient. Sometimes, data on individuals are missing, and that too can prevent the estimation of the inbreeding coefficient, which is essential for formulation of a breeding programme at the farm level and for breed development. Molecular biological approaches may solve some of these problems. RAPD (random amplified polymorphic DNA) markers use oligonucleotides to prime arbitrary amplification from genomic DNA. RAPD markers are multi-locus ones which may have the power to estimate band sharing among individuals within a breed. This sharing of bands may show the genome with levels of homozygosity between animals. This homozygosity is used to calculate the inbreeding coefficient. Band sharing in cattle has been estimated (Cho & Han, 1994; Gwakisa et al. 1994; Antoniou & Skidmore, 1995; Kantanen et al. 1995; Teale et al. 1995). Band sharing has also been used to calculate inbreeding coefficient in poultry (Kuhnlein et al. 1990; Grunder et al. 1994). In this study we report the inbreeding coefficients in cattle using band sharing between individuals within a population.
机译:近交表明群体内某基因座的纯合度。通常,近亲繁殖是根据从个体谱系计算得出的系数来估算的。但是,如果没有可用的历史记录,则无法估算近交系数。有时,缺少有关个人的数据,这也可能阻止对近交系数的估计,这对于制定农场一级的育种计划和品种开发至关重要。分子生物学方法可以解决其中一些问题。 RAPD(随机扩增的多态性DNA)标记使用寡核苷酸引发基因组DNA的任意扩增。 RAPD标记是多基因座标记,可以估计一个品种中个体之间的谱带共享。条带的这种共享可能表明基因组在动物之间具有纯合水平。该纯合性用于计算近交系数。已估计牛中的带共享(Cho&Han,1994; Gwakisa等,1994; Antonou&Skidmore,1995; Kantanen等,1995; Teale等,1995)。带共享也已用于计算家禽的近交系数(Kuhnlein等,1990; Grunder等,1994)。在这项研究中,我们使用种群内个体之间的谱带共享报告了牛的近交系数。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱:kefu@zhangqiaokeyan.com

京公网安备:11010802029741号 ICP备案号:京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有
  • 客服微信

  • 服务号