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首页> 外文期刊>Journal of dairy science >Effects of abomasal infusions of fatty acids and one-carbon donors on hepatic ceramide and phosphatidylcholine in lactating Holstein dairy cows
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Effects of abomasal infusions of fatty acids and one-carbon donors on hepatic ceramide and phosphatidylcholine in lactating Holstein dairy cows

机译：脂肪酸和单碳供体的肝芹酰胺和磷脂酰胆碱中的脂肪酸和碳供体的影响荷斯坦奶牛

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摘要

Our objectives were to (1) determine whether theabomasal infusion of behenic acid (C22:0) elevatedhepatic ceramide relative to palmitic acid (C16:0) ordocosahexaenoic acid (C22: 6n -3) infusion; (2) assesswhether the abomasal infusion of choline chloride orl-serine elevated hepatic phosphatidylcholine (PC) incows abomasally infused with C16:0; and (3) characterizethe PC lipidome in cows abomasally infused withC22: 6n -3, relative to C16:0 or C22:0 infusion. In a 5× 5 Latin square design, 5 rumen-cannulated Holsteincows (214 ± 4.9 DIM; 3.2 ± 1.1 parity) were enrolledin a study with 6-d periods. Abomasal infusates consistedof (1) palmitic acid (PA; 98% C16:0); (2) PA+ choline chloride (PA+C; 50 g/d choline chloride);(3) PA + l-serine (PA+S; 170 g/d l-serine); (4) behenicacid (BA; 92% C22:0); and (5) an algal oil rich indocosahexaenoic acid (DHA; 44% C22: 6n -3). Emulsioninfusates provided 301 g/d of total fatty acids containinga minimum of 40 g/d of C16:0. Cows were fed acorn silage-based diet. Milk was collected on d −2, −1,5, and 6. Blood was collected and liver biopsied on d 6of each period. Although we did not detect differencesin milk yield, milk fat yield and content were lower incows infused with DHA relative to PA. Plasma triacylglycerolconcentrations were lower with DHA treatmentrelative to PA or BA. Cows infused with DHA hadlower plasma insulin concentrations relative to cowsinfused with PA only. For objective 1, hepatic ceramide-d18: 2/ 22: 0 was highest in cows infused with BA relativeto other treatments. For objective 2, plasma freecholine concentrations were greater in PA+C cows relativeto PA; however, we did not observe this effect withPA+S. Plasma total PC concentrations were similarfor all treatments. Regarding the hepatic lipidome, atotal of 18 hepatic PC were higher (e.g., PC -16: 1/ 18: 2)and 25 PC were lower (e.g., PC -16: 0/ 22: 6) with PA+Cinfusion relative to PA. In addition, 17 PC were higher(e.g., PC -20: 3/ 22: 5) and 21 PC were lower (e.g., PC -18:0/ 22: 6) with PA+S infusion relative to PA. For objective3, hepatic concentrations of many individual saturatedPC (e.g., PC -18: 0/ 15: 0) were lower with DHArelative to other treatments. Hepatic concentrations ofhighly unsaturated PC with very-long-chain fatty acids(e.g., PC -14: 0/ 22: 6) were higher in DHA-infused cowsrelative to PA, PA+C, PA+S, or BA. The abomasalinfusion of emulsions containing palmitic acid, palmiticacid with choline chloride or serine, behenic acid, ordocosahexaenoic acid influence the hepatic ceramideand PC profiles of lactating cows.
机译：我们的目标是（1）确定是否山茶酸的脱毛（C22：0）升高相对于棕榈酸（C16：0）或DocosaheNoic酸（C22：6N -3）输注; （2）评估无论是氯化胆碱的脱毛输注L-丝氨酸升高的肝磷脂酰胆碱（PC）奶牛使C16融入C16：0; （3）表征牛的PC脂质组动物使牛犊脱墨C22：6N -3，相对于C16：0或C22：0输注。在5×5拉丁方形设计，5个瘤胃插管Holstein奶牛（214±4.9昏暗; 3.2±1.1奇偶校验）在6岁的研究中。脱毛鸟输注组成（1）棕榈酸（PA; 98％C16：0）; （2）PA+氯化胆碱（PA + C; 50g / d胆碱）;（3）PA + L-丝氨酸（PA + S; 170克/ D L-丝氨酸）; （4）斩首酸（Ba; 92％C22：0）; （5）富含藻类油十二碳六烯酸（DHA; 44％C22：6N -3）。乳液注入提供301g / d的总脂肪酸含有最低40克/天C16：0。奶牛被喂了一个玉米青贮饲料饮食。牛奶收集在d -2，-1，5和6.收集血液并在D 6上进行肝脏活作检每个时期。虽然我们没有发现差异在牛奶产量中，牛奶脂肪产量和含量较低奶牛与DHA相对于PA。血浆三酰基甘油DHA治疗浓度降低相对于pa或ba。与DHA融合的奶牛有相对于奶牛的降低血浆胰岛素浓度只与PA融合。对于目标1，肝神经酰胺-D18：2/22：0在与BA相对融合的奶牛中最高到其他治疗。目标2，自由等离子体PA + C奶牛相对胆碱浓度更大帕;但是，我们没有观察到这种效果PA + s。血浆总PC浓度相似所有治疗。关于肝脂肪组，a总共18只肝PC（例如，PC -16：1/18：2）BA + C，25台PC（例如，PC -16：0/22：6）较低（例如，PC -16：0/22：6）输液相对于pa。此外，17台PC更高（例如，PC -20：3/22：5）和21个PC较低（例如，PC -18：0/22：6）具有相对于PA的PA + S输注。对于目标3，许多单独的肝浓度饱和使用DHA（例如，PC -18：0/15：0）较低相对于其他治疗。肝浓度高度不饱和的PC，具有非常长的链脂肪酸（例如，PC -14：0/22：6）在DHA注入的奶牛中较高相对于PA，PA + C，PA + S或BA。阿马萨尔含棕榈酸的乳液输注棕榈酸酸胆碱或丝氨酸，山酸，或十二年六六烯酸影响肝芹酰胺和pc哺乳奶牛的个人资料。

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  • 来源
    《Journal of dairy science》 |2019年第8期|7087-7101|共15页
  • 作者

    W. A. Myers; J. E. Rico; A. N. Davis; A. B. P. Fontoura; M. J. Dineen; B. N. Tate; J. W. McFadden;

  • 作者单位

    Department of Animal Science Cornell University Ithaca NY 14853;

    Department of Animal Science Cornell University Ithaca NY 14853;

    Department of Animal Science Cornell University Ithaca NY 14853;

    Department of Animal Science Cornell University Ithaca NY 14853;

    Department of Animal Science Cornell University Ithaca NY 14853;

    Department of Animal Science Cornell University Ithaca NY 14853;

    Department of Animal Science Cornell University Ithaca NY 14853;

  • 收录信息 美国《科学引文索引》(SCI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

    behenic acid; ceramide; docosahexaenoic acid; phosphatidylcholine;

    机译：山酸;神经酰胺;十二世己烯酸;磷脂酰胆碱;

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