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首页> 外文期刊>Journal of dairy science >Identification and characterization of circular RNAs in mammary gland tissue from sheep at peak lactation and during the nonlactating period
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Identification and characterization of circular RNAs in mammary gland tissue from sheep at peak lactation and during the nonlactating period

机译：绵羊在峰值泌乳中绵羊脉冲组织圆形RNA的鉴定与表征

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摘要

Circular RNAs are a class of noncoding RNA with awidespread occurrence in eukaryote tissues, and withsome having been demonstrated to have clear biologicalfunction. In sheep, little is known about the role ofcircular RNAs in mammary gland tissue, and thereforean RNA sequencing approach was used to comparemammary gland tissue expression of circular RNAs in9 Small Tail Han sheep at peak lactation, and subsequentlywhen they were not lactating. These 9 sheephad their RNA pooled for analysis into 3 libraries frompeak lactation and 3 from the nonlactating period. Atotal of 3,278 and 1,756 circular RNAs were identifiedin the peak lactation and nonlactating mammary glandtissues, respectively, and the expression and identity of9 of them was confirmed using reverse transcriptasepolymerasechain reaction analysis and DNA sequencing.The type, chromosomal location and length of thecircular RNAs identified were ascertained. Forty upregulatedand one downregulated circular RNAs werecharacterized in the mammary gland tissue at peak lactationcompared with the nonlactating mammary glandtissue. Gene ontology enrichment analysis revealed thatthe parental genes of these differentially expressed circularRNAs were related to molecular function, binding,protein binding, ATP binding, and ion binding. Fivedifferentially expression circular RNAs were selectedfor further analysis to predict their target microRNAs,and some microRNAs reportedly associated with thedevelopment of the mammary gland were found in theconstructed circular RNA–microRNA network. Thisstudy reveals the expression profiles and characterizationof circular RNAs at 2 key stages of mammary glandactivity, thereby providing an improved understandingof the roles of circular RNAs in the mammary gland ofsheep.
机译：圆形rnas是一类与a的非致rna在真核组织中普遍发生，以及有些人被证明是明确的生物学功能。在羊，很少是众所周知的作用乳腺组织中的圆形RNA，因此使用RNA测序方法进行比较圆形RNA的乳腺组织表达9个小尾汉羊在峰值哺乳期，随后当他们没有哺乳期的时候。这9只羊他们的RNA汇集为分析3个图书馆峰值哺乳期和3来自非触发期。一种确定总共3,278和1,756个圆形RNA在峰值哺乳期和非乳液乳腺中分别是组织，以及表达和身份使用逆转录淀粉基甲戊聚糖酶确认它们中的9个链反应分析和DNA测序。类型，染色体位置和长度确定了圆形的RNA。四十个上调并且一个下调的圆形RNA是在峰值哺乳期的特征在乳腺组织中与非乳液乳腺相比组织。基因本体富集分析显示这些差异表达圆形的父母基因RNA与分子功能有关，结合，蛋白质结合，ATP结合和离子结合。五选择差异表达圆形RNA为了进一步分析以预测其目标小罗车，和一些microRNAS据说与之相关联在乳腺的发展中被发现构建圆形RNA-MicroRNA网络。这研究揭示了表达谱和表征在乳腺2个关键阶段的圆形RNA活动，从而提供了改进的理解圆形RNA在乳腺中的作用羊。

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  • 来源
    《Journal of dairy science》 |2021年第2期|2396-2409|共14页
  • 作者

    Jiqing Wang; Huitong Zhou; Jon G. H. Hickford; Zhiyun Hao; Hua Gong; Jiang Hu; Xiu Liu; Shaobin Li; Jiyuan Shen; Na Ke; Yize Song; Lirong Qiao; Yuzhu Luo;

  • 作者单位

    Gansu Key Laboratory of Herbivorous Animal Biotechnology Faculty of Animal Science and Technology Gansu Agricultural University Lanzhou 730070 China;

    Gansu Key Laboratory of Herbivorous Animal Biotechnology Faculty of Animal Science and Technology Gansu Agricultural University Lanzhou 730070 China Gene-Marker Laboratory Faculty of Agriculture and Life Sciences Lincoln University Lincoln 7647 New Zealand;

    Gansu Key Laboratory of Herbivorous Animal Biotechnology Faculty of Animal Science and Technology Gansu Agricultural University Lanzhou 730070 China Gene-Marker Laboratory Faculty of Agriculture and Life Sciences Lincoln University Lincoln 7647 New Zealand;

    Gansu Key Laboratory of Herbivorous Animal Biotechnology Faculty of Animal Science and Technology Gansu Agricultural University Lanzhou 730070 China;

    Gansu Key Laboratory of Herbivorous Animal Biotechnology Faculty of Animal Science and Technology Gansu Agricultural University Lanzhou 730070 China Gene-Marker Laboratory Faculty of Agriculture and Life Sciences Lincoln University Lincoln 7647 New Zealand;

    Gansu Key Laboratory of Herbivorous Animal Biotechnology Faculty of Animal Science and Technology Gansu Agricultural University Lanzhou 730070 China;

    Gansu Key Laboratory of Herbivorous Animal Biotechnology Faculty of Animal Science and Technology Gansu Agricultural University Lanzhou 730070 China;

    Gansu Key Laboratory of Herbivorous Animal Biotechnology Faculty of Animal Science and Technology Gansu Agricultural University Lanzhou 730070 China;

    Gansu Key Laboratory of Herbivorous Animal Biotechnology Faculty of Animal Science and Technology Gansu Agricultural University Lanzhou 730070 China;

    Gansu Key Laboratory of Herbivorous Animal Biotechnology Faculty of Animal Science and Technology Gansu Agricultural University Lanzhou 730070 China;

    Gansu Key Laboratory of Herbivorous Animal Biotechnology Faculty of Animal Science and Technology Gansu Agricultural University Lanzhou 730070 China;

    Gansu Key Laboratory of Herbivorous Animal Biotechnology Faculty of Animal Science and Technology Gansu Agricultural University Lanzhou 730070 China;

    Gansu Key Laboratory of Herbivorous Animal Biotechnology Faculty of Animal Science and Technology Gansu Agricultural University Lanzhou 730070 China;

  • 收录信息 美国《科学引文索引》(SCI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

    circular RNA; mammary gland; sheep; RNA sequencing;

    机译：圆形RNA;乳腺;羊;RNA测序;

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