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首页> 外文期刊>Journal of dairy science >Large-scale preparation and glycan characterization of sialylglycopeptide from bovine milk glycomacropeptide and its bifidogenic properties
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Large-scale preparation and glycan characterization of sialylglycopeptide from bovine milk glycomacropeptide and its bifidogenic properties

机译：牛奶糖肽的大规模制备及甘草表征牛奶糖肽及其双歧膜性能

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摘要

Bovine glycomacropeptide (GMP) is a 7,000-Daglycopolypeptide released from κ-casein during cheesemaking. The O-glycan chains linked to GMP havemany biological activities, but their utilization for nutraceuticalproducts is limited due to their low content.To concentrate the functional glycan chains of GMP,we prepared sialylglycopeptide concentrate (SGC) fromGMP-containing whey protein concentrate via proteolyticdigestion of peptide chains and concentration ofsialylglycopeptide by ultrafiltration using membraneswith a molecular weight cut-off of 1,000 Da. The abundantsaccharides detected in the prepared SGC wereN-acetylneuraminic acid (Neu5Ac: 32.3% wt/wt), Nacetylgalactosamine(11.3%), and galactose (10.2%),which constitute O-glycans attached to GMP. TheNeu5Ac content in SGC was found concentrated at approximately4.8-fold of its content in GMP-containingwhey protein concentrate (6.8%). Structural analysisof O-glycopeptides by liquid chromatography tandemmass spectrometry identified 88 O-glycopeptides.Moreover, O-acetylated or O-diacetylated Neu5Ac wasdetected in addition to the previously characterizedO-glycans of GMP. Quantitative analysis of O-glycanin SGC by fluorescence labeling of chemically releasedO-glycan revealed that a disialylated tetrasaccharidewas the most abundant glycan (76.6% of the total Oglycan).We further examined bifidogenic properties ofSGC in vitro, which revealed that SGC served as amore potent carbon source than GMP and contributesto the growth-promoting effects on certain species ofbifidobacteria. Overall, our study findings indicate thatSGC contains abundant O-glycans and has a bifidogenicactivity. Moreover, the protocol for the preparation ofSGC described herein is relatively simple, providing ahigh yield of glycan, and can be used for large-scalepreparation.
机译：牛甘蔗肽（GMP）是7,000岁在奶酪期间从κ-酪蛋白释放的糖肽制作。与GMP相关的O-Glycan链有许多生物活动，但它们对营养保健品的利用由于其含量低，产品受限。浓缩GMP的功能性甘草链，我们制备SiaLylglycopeptide浓缩物（SGC）来自含GMP的乳清蛋白通过蛋白水解浓缩消化肽链和浓度通过使用膜超滤的唾液酸肽分子量截止1,000 da。丰富在制备的SGC中检测到糖类是N-乙酰尿氨酸（Neu5Ac：32.3％wt / wt），甲酰键酰胺胺（11.3％）和半乳糖（10.2％），构成与GMP附加的O-聚糖。这发现SGC中的Neu5Ac含量在大约浓缩含GMP的4.8倍的内容乳清蛋白浓缩物（6.8％）。结构分析通过液相色谱串联的O-糖肽质谱鉴定出88 o-糖肽。此外，O-乙酰化或O-二乙酰化Neu5ac是除了先前的特征之外还检测到GMP的o-聚糖。 O-Glycan的定量分析通过化学释放的荧光标记在SGC中O-甘草透露，一种松弛四糖是最丰富的甘草（oglycan的76.6％）。我们进一步研究了双歧膜的性质SGC体外，显示SGC作为一个比GMP更多的碳来源并有助于对某些物种的增长促进影响双歧杆菌。总体而言，我们的研究结果表明SGC含有丰富的O-聚糖，具有双歧糖性活动。此外，制备的方案本文描述的SGC相对简单，提供了一个甘草的高产率，可用于大规模准备。

著录项

  • 来源
    《Journal of dairy science》 |2021年第2期|1433-1444|共12页
  • 作者

    Hirofumi Fukudome; Toshiyuki Yamaguchi; Junichi Higuchi; Akihiro Ogawa; Yuichi Taguchi; Juan Li; Toshihide Kabuki; Koutaro Ito; Fumihiko Sakai;

  • 作者单位

    Milk Science Research Institute Megmilk Snow Brand Co. Ltd. 1-1-2 Minamidai Kawagoe-shi Saitama 350-1165 Japan;

    Milk Science Research Institute Megmilk Snow Brand Co. Ltd. 1-1-2 Minamidai Kawagoe-shi Saitama 350-1165 Japan;

    Milk Science Research Institute Megmilk Snow Brand Co. Ltd. 1-1-2 Minamidai Kawagoe-shi Saitama 350-1165 Japan;

    Milk Science Research Institute Megmilk Snow Brand Co. Ltd. 1-1-2 Minamidai Kawagoe-shi Saitama 350-1165 Japan;

    Milk Science Research Institute Megmilk Snow Brand Co. Ltd. 1-1-2 Minamidai Kawagoe-shi Saitama 350-1165 Japan;

    Milk Science Research Institute Megmilk Snow Brand Co. Ltd. 1-1-2 Minamidai Kawagoe-shi Saitama 350-1165 Japan;

    Milk Science Research Institute Megmilk Snow Brand Co. Ltd. 1-1-2 Minamidai Kawagoe-shi Saitama 350-1165 Japan;

    Milk Science Research Institute Megmilk Snow Brand Co. Ltd. 1-1-2 Minamidai Kawagoe-shi Saitama 350-1165 Japan;

    Milk Science Research Institute Megmilk Snow Brand Co. Ltd. 1-1-2 Minamidai Kawagoe-shi Saitama 350-1165 Japan;

  • 收录信息 美国《科学引文索引》(SCI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

    glycomacropeptide; O-glycan; Neu5Ac; sialylglycopeptide; bifidobacteria;

    机译：GlycomaCropeptide;o-glycan;neu5ac;SiaLylglycopeptide;双歧杆菌;

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