ABSTRACT Subacute ruminal acidosis affects fermentation and endotoxin concentration in the rumen and relative expression of the CD14ITLR4IMD2 genes involved in lipopolysaccharide systemic immune response in dairy cows
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首页> 外文期刊>Journal of dairy science >Subacute ruminal acidosis affects fermentation and endotoxin concentration in the rumen and relative expression of the CD14ITLR4IMD2 genes involved in lipopolysaccharide systemic immune response in dairy cows
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Subacute ruminal acidosis affects fermentation and endotoxin concentration in the rumen and relative expression of the CD14ITLR4IMD2 genes involved in lipopolysaccharide systemic immune response in dairy cows

机译：亚急性瘤胃酸中毒会影响瘤胃中的发酵和内毒素浓度以及参与奶牛脂多糖全身免疫反应的CD14ITLR4IMD2基因的相对表达

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摘要

ABSTRACTThe first objective of this study was to investigate the effects of subacute ruminal acidosis (SARA) on fermentation, ruminal free lipopolysaccharides (LPS), and expression of the cluster of differentiation 14 (CD14), toll-like receptor 4 (TLR4), and myeloid differentiation protein 2 (MD2) complex in white blood cells involved in the systemic immune response in dairy cows. The second objective was a study of whether increased expression of the LPS receptor complex led to increases in the concentrations of plasma high-density lipoprotein (HDL) and serum Ca. Three hundred five dairy cows located in 13 Polish high-yielding dairy commercial farms were selected according to their days in milk (40–150 d; average = 75), 305-d milk yield (10,070–12,041 kg; average = 10,940), and number of lactations (primiparous, n = 139 and multiparous, n = 166). Next, the herds were segregated into 3 groups based on the percentages of cows with an assigned value of ruminal fluid pH: SARA-positive, SARA-risk, and SARA-negative herds. Moreover, 305 selected dairy cows were divided according to the classification based on ruminal fluid pH into 3 groups as healthy (pH >5.81), risk (pH 5.8–5.6) and acidotic cows (pH <5.6). Rumen fluid samples were collected via rumenocentesis. In the AC group, we recorded higher concentrations of ruminal free LPS [4.57 Log10endotoxin units (EU)/mL; 42,206 EU/mL] compared with the healthy group (4.48 Log10EU/mL; 34,179 EU/mL). Similarly, the concentration of ruminal free LPS was higher in SARA-positive herds (4.60 Log10EU/mL; 43,000 EU/mL) compared with SARA-negative herds (4.47 Log10EU/mL; 32,225 EU/mL). The relative mRNA abundance of genes associated with the function of LPS receptors, such asCD14,TLR4, andMD2, in white blood cells differed between all experimental groups on both cow and herd levels. In the acidotic group, we recorded higher concentrations of HDL (78.16 vs. 68.32 mg/dL) and serum amyloid A (10.80 vs. 9.16 µg/mL) and lower concentrations of Ca (8.26 vs. 10.16 mg/dL) and haptoglobin (470.19 vs. 516.85 ng/mL) compared with the healthy group. Similar results were obtained in the SARA herd status analysis, but the concentration of lipopolysaccharide-binding protein differed statistically. Moreover, the pH of ruminal fluid was negatively correlated with relative mRNA abundance of genes such asCD14,TLR4,MD2, and concentrations of serum HDL and serum amyloid A, although positively correlated with serum Ca. The results indicated that decreases in ruminal fluid pH increased the release of free LPS into the rumen and stimulated the expression of the LPS receptor complex and immune response. Moreover, an increase in the expression of the LPS receptor led to higher concentrations of plasma HDL and lower serum Ca, which may be a protective mechanism against endotoxemia. However, the biological significance of these results needs to be investigated further in larger field trials.
机译： 抽象 这项研究的首要目的是研究亚急性瘤胃酸中毒（SARA）对发酵，瘤胃游离脂多糖（LPS）和分化簇表达的影响14（ CD14 ），toll​​样受体4（ TLR4 ）和髓样分化蛋白2（ MD2 ）复合物中的白细胞参与了奶牛的全身免疫反应。第二个目标是研究LPS受体复合物的表达增加是否导致血浆高密度脂蛋白（HDL）和血清Ca浓度增加。根据它们在牛奶中的存续天数（40–150 d；平均= 75），305 d产奶量（10,070–12,041公斤；平均= 10,940），选择了位于13个波兰高产奶牛商业农场中的305头奶牛，和泌乳次数（初产，n = 139，多胎，n = 166）。接下来，根据具有瘤胃液pH值指定值的母牛的百分比，将牛群分为3组：SARA阳性，SARA高风险和SARA阴性。此外，根据瘤胃液pH值，将305头选定的奶牛根据其分类分为健康（pH> 5.81），高危（pH 5.8-5.6）和酸中毒（pH <5.6）三组。通过瘤胃穿刺术收集瘤胃液样品。在AC组中，我们记录了更高浓度的瘤胃游离LPS [4.57 Log 10 内毒素单位（EU）/ mL； 42,206 EU / mL]与健康组相比（4.48 Log 10 EU / mL; 34,179 EU / mL）。同样，与SARA阴性牛群相比，SARA阳性牛群的瘤胃游离LPS浓度更高（4.60 Log 10 EU / mL； 43,000 EU / mL） （4.47 Log 10 EU / mL; 32,225 EU / mL）。与LPS受体功能相关的基因的相对mRNA丰度，例如 CD14 ， TLR4 和 MD2 < / ce：italic>，所有实验组的白细胞在牛和牛的水平上均不同。在酸中毒组中，我们记录到较高的HDL（78.16 vs. 68.32 mg / dL）和血清淀粉样蛋白A（10.80 vs. 9.16 µg / mL）和较低的Ca（8.26 vs. 10.16 mg / dL）和触珠蛋白（ 470.19比516.85 ng / mL）。在SARA牛群状态分析中获得了相似的结果，但是脂多糖结合蛋白的浓度在统计学上有所不同。此外，瘤胃液的pH值与 CD14 ， TLR4 ， MD2等基因的相对mRNA丰度呈负相关。 ，以及血清HDL和血清淀粉样蛋白A的浓度，尽管与血清Ca呈正相关。结果表明，瘤胃液pH的降低会增加游离LPS向瘤胃的释放，并刺激LPS受体复合物的表达和免疫反应。此外，LPS受体表达的增加导致血浆HDL浓度升高和血清Ca降低，这可能是针对内毒素血症的保护机制。但是，这些结果的生物学意义需要在更大的现场试验中进一步研究。

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  • 来源
    《Journal of dairy science》 |2018年第2期|1297-1310|共14页
  • 作者

    B. Stefanska; W. Człapa; E. Pruszynska-Oszmałek; D. Szczepankiewicz; V. Fievez; J. Komisarek; K. Stajek; W. Nowak;

  • 作者单位

    Department of Animal Nutrition and Feed Management, Poznań University of Life Science;

    Department of Animal Nutrition and Feed Management, Poznań University of Life Science;

    Department of Animal Physiology and Biochemistry, Faculty of Animal Science, Poznań University of Life Science;

    Department of Animal Physiology and Biochemistry, Faculty of Animal Science, Poznań University of Life Science;

    Laboratory for Animal Nutrition and Animal Product Quality, Campus Coupure BW13;

    Department of Animal Breeding and Product Quality Assessment, Poznań University of Life Sciences, Złotniki;

    Department of Animal Nutrition and Feed Management, Poznań University of Life Science;

    Department of Animal Nutrition and Feed Management, Poznań University of Life Science;

  • 收录信息 美国《科学引文索引》(SCI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

    rumenocentesis; LPS; gene expression; inflammation;

    机译：瘤胃穿刺;LPS;基因表达;炎症;

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