...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Journal of dairy science >Body condition score and plane of nutrition prepartum affect adipose tissue transcriptome regulators of metabolism and inflammation in grazing dairy cows during the transition period
【24h】

Body condition score and plane of nutrition prepartum affect adipose tissue transcriptome regulators of metabolism and inflammation in grazing dairy cows during the transition period

机译：过渡期间，身体状况评分和营养性产前平面影响放牧奶牛的脂肪组织转录组调节代谢和炎症

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Recent studies demonstrating a higher incidence of metabolic disorders after calving have challenged the management practice of increasing dietary energy density during the last ～3 wk prepartum. Despite our knowledge at the whole-animal level, the tissue-level mechanisms that are altered in response to feeding management prepartum remain unclear. Our hypothesis was that prepartum body condition score (BCS), in combination with feeding management, plays a central role in the peripartum changes associated with energy balance and inflammatory state. Twenty-eight mid-lactation grazing dairy cows of mixed age and breed were randomly allocated to 1 of 4 treatment groups in a 2 × 2 factorial arrangement: 2 prepartum BCS categories (4.0 and 5.0, based on a 10-point scale; BCS4, BCS5) obtained via differential feeding management during late-lactation, and 2 levels of energy intake during the 3 wk preceding calving (75 and 125% of estimated requirements). Subcutaneous adipose tissue was harvested via biopsy at -1, 1, and 4 wk relative to parturition. Quantitative polymerase chain reaction was used to measure mRNA and microRNA (miRNA) expression of targets related to fatty acid metabolism (lipogenesis, lipolysis), adipokine synthesis, and inflammation. Both prepartum BCS and feeding management had a significant effect on mRNA and miRNA expression throughout the peripartum period. Overfed BCS5 cows had the greatest prepartum expression of fatty acid synthase (FASN) and an overall greater expression of leptin (LEP); BCS5 was also associated with greater overall adiponectin (ADIPOQ) and peroxisome proliferator-activated receptor gamma (PPARG), whereas overfeeding upregulated expression of proad-ipogenic miRNA. Higher postpartum expression of che-mokine ligand 5 (CCL5) and the cytokines interleukin 6 (IL6) and tumor necrosis factor (TNF) was detected in overfed BCS5 cows. Feed-restricted BCS4 cows had the highest overall interleukin 1 (IL1B) expression. Prepartum feed restriction resulted in greater chemo-kine ligand 2 (CCL2) expression. Overall, changes in mRNA expression were consistent with the expression pattern of inflammation-related miRNA. These data shed light on molecular mechanisms underlying the effect of prepartum BCS and feeding management on metabolic and inflammatory status of adipose tissue during the peripartum period. Data support the use of a controlled feed restriction prepartum in optimally conditioned cows, as well as the use of a higher level of dietary energy in under-conditioned cows.
机译：最近的研究表明，产犊后代谢紊乱的发生率更高，这已经挑战了在产后最后3周内增加饮食能量密度的管理方法。尽管我们了解全动物水平，但仍不清楚因产前喂养管理而改变的组织水平机制。我们的假设是，产前身体状况评分（BCS）与喂养管理相结合，在与能量平衡和炎症状态相关的围产期变化中起着核心作用。将28头不同年龄和不同品种的哺乳中期奶牛以2×2因子分布随机分配到4个治疗组中的1个：2个产前BCS类别（4.0和5.0，基于10分制； BCS4， BCS5）是在泌乳后期通过差异饲喂管理获得的，而产犊前3周则摄入了2级能量（估计需求量的75％和125％）。相对于分娩，在-1、1和4周通过活检收集皮下脂肪组织。定量聚合酶链反应用于测量与脂肪酸代谢（脂肪生成，脂肪分解），脂肪因子合成和炎症相关的靶标的mRNA和microRNA（miRNA）表达。产前BCS和喂养管理对整个围产期的mRNA和miRNA表达均具有显着影响。饲喂过量的BCS5奶牛的产前脂肪酸合成酶（FASN）表达最高，而瘦素（LEP）总体表达最高； BCS5还与总体脂联素（ADIPOQ）和过氧化物酶体增殖物激活的受体伽玛（PPARG）更高有关，而摄食促成脂miRNA的表达却上调了。在饲喂过量的BCS5奶牛中发现了che-mokine配体5（CCL5）和细胞因子白介素6（IL6）和肿瘤坏死因子（TNF）的较高产后表达。饲料限制的BCS4奶牛的白介素1（IL1B）总体表达最高。产前饲料限制导致更高的化学因子配体2（CCL2）表达。总体而言，mRNA表达的变化与炎症相关miRNA的表达模式一致。这些数据揭示了围产期BCS和进食管理对脂肪组织代谢和炎症状态潜在影响的分子机制。数据支持在条件最佳的奶牛中使用受控的饲料限制产前，以及条件不足的奶牛中使用更高水平的饮食能量。

著录项

  • 来源
    《Journal of dairy science》 |2016年第1期|758-770|共13页
  • 作者

    M. Vailati-Riboni; M. Kanwal; O. Bulgari; S. Meier; N. V. Priest; C. R. Burke; J. K. Kay; S. McDougall; M. D. Mitchell; C. G. Walker; M. Crookenden; A. Heiser; J. R. Roche; J. J. Loor;

  • 作者单位

    Department of Animal Sciences, University of Illinois, Urbana 61801;

    Department of Animal Sciences, University of Illinois, Urbana 61801;

    Department of Animal Sciences, University of Illinois, Urbana 61801,Department of Molecular and Translational Medicine, University of Brescia, Brescia 25123, Italy;

    DairyNZ Limited, Private Bag 3221, Hamilton 3240, New Zealand;

    DairyNZ Limited, Private Bag 3221, Hamilton 3240, New Zealand;

    DairyNZ Limited, Private Bag 3221, Hamilton 3240, New Zealand;

    DairyNZ Limited, Private Bag 3221, Hamilton 3240, New Zealand;

    Cognosco, Anexa Animal Health, PO Box 21, Morrinsville 3300, New Zealand;

    University of Queensland, Centre for Clinical Research, Royal Brisbane & Women's Hospital Campus, Herston, Queensland 4029, Australia;

    DairyNZ Limited, Private Bag 3221, Hamilton 3240, New Zealand;

    DairyNZ Limited, Private Bag 3221, Hamilton 3240, New Zealand;

    AgResearch, Hopkirk Research Institute, Grasslands Research Centre, Palmerston North 4442, New Zealand;

    DairyNZ Limited, Private Bag 3221, Hamilton 3240, New Zealand;

    Department of Animal Sciences, University of Illinois, Urbana 61801;

  • 收录信息 美国《科学引文索引》(SCI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

    transition cow; nutrition; inflammation; immune response;

    机译：过渡牛营养;炎;免疫反应;

相似文献

  • 外文文献
  • 中文文献
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号