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首页> 外文期刊>Journal of dairy science >Effect of increasing body condition on key regulators of fat metabolism in subcutaneous adipose tissue depot and circulation of nonlactating dairy cows
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Effect of increasing body condition on key regulators of fat metabolism in subcutaneous adipose tissue depot and circulation of nonlactating dairy cows

机译：身体状况的增强对皮下脂肪组织贮藏所脂肪代谢和非泌乳奶牛血液循环关键调节因子的影响

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摘要

In response to negative energy balance, overcondi-tioned cows mobilize more body fat than thin cows and subsequently are prone to develop metabolic disorders. Changes in adipose tissue (AT) metabolism are barely investigated in overconditioned cows. Therefore, the objective was to investigate the effect of increasing body condition on key regulator proteins of fat metabolism in subcutaneous AT and circulation of dairy cows. Nonlactating, nonpregnant dairy cows (n = 8) investigated in the current study served as a model to elucidate the changes in the course of overcondition independent from physiological changes related to ges-tation, parturition, and lactation. Cows were fed diets with increasing portions of concentrate during the first 6 wk of the experiment until 60% were reached, which was maintained for 9 wk. Biopsy samples from AT of the subcutaneous tailhead region were collected every 8 wk, whereas blood was sampled monthly. Within the experimental period cows had an average BW gain of 243 ± 33.3 kg. Leptin and insulin concentrations were increased until wk 12. Based on serum concentrations of glucose, insulin, and nonesterified fatty acids, the surrogate indices for insulin sensitivity were calculated. High-concentrate feeding led to decreased quantitative insulin sensitivity check index and homeostasis model assessment due to high insulin and glucose concentrations indicating decreased insulin sensitivity. Adiponectin, an adipokine-promoting insulin sensi- tivity, decreased in subcutaneous AT, but remained unchanged in the circulation. The high-concentrate diet affected key enzymes reflecting AT metabolism such as AMP-activated protein kinase and hormone-sensitive lipase, both represented as the proportion of the phosphorylated protein to total protein, as well as fatty acid synthase. The extent of phosphorylation of AMP-activated protein kinase and the protein expression of fatty acid synthase were inversely regulated throughout the experimental period, whereas the extent of phosphorylation of hormone-sensitive lipase was consistently decreasing by the high-concentrate diet. Overcondition in nonpregnant, nonlactating dairy cows changed the expression of key regulator proteins of AT metabolism and circulation accompanied by impaired insulin sensitivity, which might increase the risk for metabolic disorders.
机译：为了应对负能量平衡，过度适应的母牛比瘦母牛动员的体内脂肪更多，因此容易发生代谢紊乱。在条件过高的母牛中，脂肪组织（AT）代谢的变化几乎没有得到研究。因此，目的是研究增加身体状况对皮下AT和奶牛循环中脂肪代谢关键调节蛋白的影响。在本研究中研究的非泌乳，非妊娠奶牛（n = 8）用作阐明过度状态过程中的变化的模型，其独立于与妊娠，分娩和泌乳相关的生理变化。在实验的前6周期间，向母牛饲喂的饲料中增加了浓缩物的比例，直至达到60％，并保持9周。每8周从皮下尾部区域的AT采集活检样本，而每月采血。在实验期内，奶牛的平均体重增加了243±33.3公斤。瘦素和胰岛素的浓度一直增加到12周。根据血清葡萄糖，胰岛素和非酯化脂肪酸的浓度，计算出胰岛素敏感性的替代指标。高浓度喂养导致定量胰岛素敏感性检查指数和稳态模型评估降低，原因是胰岛素和葡萄糖浓度高表明胰岛素敏感性降低。脂联素是一种促进脂肪因子的胰岛素敏感性，在皮下AT下降，但在循环中保持不变。高浓度饮食影响了反映AT代谢的关键酶，例如AMP激活的蛋白激酶和激素敏感的脂肪酶，两者均表示为磷酸化蛋白与总蛋白的比例，以及脂肪酸合酶。在整个实验期间，AMP激活的蛋白激酶的磷酸化程度和脂肪酸合酶的蛋白质表达均受到反调节，而高浓度饮食则使激素敏感性脂肪酶的磷酸化程度持续降低。非妊娠，非哺乳期奶牛的过度适应改变了AT代谢和循环的关键调节蛋白的表达，并伴有胰岛素敏感性受损，这可能会增加代谢紊乱的风险。

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  • 来源
    《Journal of dairy science》 |2015年第2期|1057-1068|共12页
  • 作者

    L. Locher; S. Haeussler; L. Laubenthal; S. P. Singh; J. Winkler; A. Kinoshita; A. Kenez; J. Rehage; K. Huber; H. Sauerwein; S. Daenicke;

  • 作者单位

    Clinic for Cattle, University of Veterinary Medicine Hannover, Foundation, 30173 Hannover, Germany,Center of Veterinary Clinical Medicine, LMU Munich, Sonnenstrasse 16, 85764 Oberschleissheim, Germany;

    Institute of Animal Science, Physiology & Hygiene Unit, University of Bonn, 53115 Bonn, Germany;

    Institute of Animal Science, Physiology & Hygiene Unit, University of Bonn, 53115 Bonn, Germany;

    Institute of Animal Science, Physiology & Hygiene Unit, University of Bonn, 53115 Bonn, Germany,Physiology, Reproduction and Shelter Management Division, Central Institute for Research on Goats, Makhdoom, Farah 281 122, Mathura (U.P.), India;

    Institute of Animal Nutrition, Friedrich-Loeffler Institute (FLI), Federal Research Institute for Animal Health, 38116 Braunschweig, Germany;

    Clinic for Cattle, University of Veterinary Medicine Hannover, Foundation, 30173 Hannover, Germany;

    Department of Physiology, University of Veterinary Medicine Hannover, Foundation, 30173 Hannover, Germany;

    Clinic for Cattle, University of Veterinary Medicine Hannover, Foundation, 30173 Hannover, Germany;

    Department of Physiology, University of Veterinary Medicine Hannover, Foundation, 30173 Hannover, Germany;

    Institute of Animal Science, Physiology & Hygiene Unit, University of Bonn, 53115 Bonn, Germany;

    Institute of Animal Nutrition, Friedrich-Loeffler Institute (FLI), Federal Research Institute for Animal Health, 38116 Braunschweig, Germany;

  • 收录信息 美国《科学引文索引》(SCI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

    fat metabolism; dairy cow; subcutaneous adipose tissue;

    机译：脂肪代谢奶牛;皮下脂肪组织;

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