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首页> 外文期刊>Journal of dairy science >Hot topic: Successful fixed-time insemination within 21 d after first insemination by combining chemical pregnancy diagnosis on d 18 with a rapid resynchronization program
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Hot topic: Successful fixed-time insemination within 21 d after first insemination by combining chemical pregnancy diagnosis on d 18 with a rapid resynchronization program

机译:热门话题:通过将第18天的化学妊娠诊断与快速重新同步程序相结合,在首次人工授精后21 d内成功进行了固定时间授精

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摘要

Cattle that are not pregnant to first fixed-time artificial insemination (TAI) may be resynchronized for a second TAI if they are found nonpregnant at pregnancy diagnosis. The specific interval between first and second TAI ranges from 4 to 8 wk. The selected interval depends on the available method of pregnancy diagnosis and the efficiency of the resynchronization program. The objective of this experiment was to evaluate a pregnancy diagnosis and resynchronization system that achieved a 21-d interval between TAI. This 21-d interval approximates the natural return-to-service interval. It also enables resynchronization to be implemented within the same estrous cycle in which cattle are first inseminated. Holstein heifers were randomly assigned to a 21-d resynchronization program (21d_resynch; n = 40) or a control group in which estrus was observed for the purpose of re-insemination (control; n = 29). The 21d_resynch heifers were diagnosed for pregnancy on d 18 after a TAI (d 0) by using predetermined cutoff values for 2'-5' oligoadenylate synthetase 1 (Oasl) gene expression in leukocytes and plasma progesterone concentration. Heifers that were not pregnant to first TAI had greater expression of Oasl at the time of PGF_(2α) (d -3) than pregnant heifers, but this relationship was reversed on d 18 after TAI: the heifers that were pregnant to first TAI had almost 5-fold greater expression of Oasl compared with nonpregnant heifers. Nonpregnant heifers in the 21d_resynch group were injected with a luteolytic dose of PGF_(2α) on d 19 and were injected with GnRH on d 21 and submitted to TAI. The pregnancy per AI after first insemination was similar for 21d_resynch (50.0%; pregnancy diagnosis on d 18) and control (51.7%; pregnancy diagnosis on d 27). Likewise, no difference was detected in second insemination pregnancy per AI for 21d_resynch (36.8%; nonpregnant heifers TAI on d 21) and control (35.7%; nonpregnant heifers inseminated at return to estrus or after nonpregnant diagnosis on d 27). The interval between first and second insemination was shorter for 21d_resynch compared with control (21.0 ± 0 and 27.5 ± 2.1 d). The conclusion is that a TAI resynchronization can be programmed within 21 d of previous TAI when a d 18 pregnancy test and a rapid resynchronization are used.
机译:如果未在怀孕固定时间进行人工授精(TAI),未怀孕的牛在怀孕诊断时被发现未怀孕,则可以将其重新同步进行第二次TAI。第一和第二TAI之间的特定间隔范围是4到8 wk。选择的时间间隔取决于怀孕诊断的可用方法和重新同步程序的效率。该实验的目的是评估在TAI之间达到21天间隔的妊娠诊断和再同步系统。这个21天的时间间隔近似于自然的返回服务间隔。它还使重新同步可以在牛首次受精的同一发情周期内实现。荷斯坦小母牛被随机分配到21天再同步程序(21d_resynch; n = 40)或对照组,在该组中观察到发情以重新授精(对照组; n = 29)。通过使用预定的白细胞中2'-5'寡腺苷酸合成酶1(Oasl)基因表达的截止值和血浆孕酮浓度,在TAI后第18天诊断出21d_resynch小母牛怀孕。没有怀孕的小母牛在PGF_(2α)(d -3)时比怀孕的小母牛具有更高的Oasl表达,但是这种关系在TAI后的第18天被逆转:怀孕的母牛在第一次TAI时具有与未怀孕的小母牛相比,Oasl的表达增加了近5倍。在21d_resynch组中,未怀孕的小母牛在第19天注射了黄体溶解剂量的PGF_(2α),在第21天注射了GnRH,并送交TAI。初次授精后每个AI的妊娠率在21d_resynch(50.0%;在第18天的妊娠诊断)和对照(51.7%;在第27天的妊娠诊断)相似。同样,在21d_resynch(36.8%;未怀孕的小母牛TAI在第21天)和对照(35.7%;在发情期或未怀孕的诊断后第27天的授精的未怀孕小母牛)中,每个AI的第二次受精怀孕也没有差异。与对照组相比,第一次授精和第二次授精之间的间隔较短,为21d_resynch(21.0±0和27.5±2.1 d)。结论是,当使用d 18妊娠试验和快速重新同步时,可以在先前TAI的21 d内对TAI重新同步进行编程。

著录项

  • 来源
    《Journal of dairy science》 |2010年第12期|p.5668-5672|共5页
  • 作者单位

    Division of Animal Science, University of Missouri, Columbia 65211;

    Division of Animal Science, University of Missouri, Columbia 65211;

    Division of Animal Science, University of Missouri, Columbia 65211;

    Division of Animal Science, University of Missouri, Columbia 65211;

    Division of Animal Science, University of Missouri, Columbia 65211;

  • 收录信息 美国《科学引文索引》(SCI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

    oasl; pregnancy; resynchronization; heifer;

    机译:绿洲怀孕;重新同步小母牛;

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