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首页> 外文期刊>Journal of Virology >Adenovirus transcription. IV. Synthesis of viral-specific RNA in human cells infected with temperature-sensitive mutants of adenovirus 5.
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Adenovirus transcription. IV. Synthesis of viral-specific RNA in human cells infected with temperature-sensitive mutants of adenovirus 5.

机译：腺病毒转录。 IV。用腺病毒5的温度敏感突变体感染人细胞中病毒特异性RNA的合成。

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摘要

Cytoplasmic RNA sequences produced in HeLa cells infected with the adeno-virus 5 temperature-sensitive mutants ts1, ts2, ts9, ts17, ts18, ts19, ts20, ts22, ts49, ts36, and ts125 were characterized by hybridization to DNA probes generated by strand separation of restriction endonuclease fragments of adenovirus 5 DNA. Two ""early'' mutants defective in DNA synthesis, ts125 and ts36, fail to make wild-type levels of all previously reported classes of late RNA at the nonpermissive temperature. At 40.5 degrees C, both ts125 and ts36 synthesize a wild-type complement of early cytoplasmic RNA 16 h after infection. Under these conditions, no ""late'' cytoplasmic RNA sequences were observed. Similarly, nuclear RNA present in these cells resembled early cytoplasmic RNA rather than late nuclear RNA. All the late adenovirus 5 temperature-sensitive mutants synthesized normal wild-type levels of late cytoplasmic RNA at the nonpermissive temperature, except ts2, which appears to overproduce certain cytoplasmic species.
机译：在感染的HeLa细胞中产生的细胞质RNA序列感染腺瘤病毒5温度敏感突变体TS1，TS2，TS9，TS17，TS18，TS19，TS20，TS22，TS49，TS36和TS125的特征在于与股线产生的DNA探针杂交腺病毒5 DNA的限制性内切核酸酶的分离。在DNA合成中有两个“早期”突变体，TS125和TS36缺陷，未能在非智能温度下使所有先前报告的晚期RNA的野生型水平。在40.5℃下，TS125和TS36均在感染后合成早期细胞质RNA的野生型补体。在这些条件下，未观察到“”迟到的“细胞质RNA序列。类似地，这些细胞中存在的核RNA类似于早期细胞质RNA而不是晚期核RNA。所有晚期腺病毒5温度敏感突变体在非智能温度下合成正常野生型水平的晚期细胞质RNA，除了TS2，似乎过度提出了某些细胞质物种。

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