Isolation and characterization of outer membrane permeability mutants in Escherichia coli K-12.

S A Benson; A Decloux

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Isolation and characterization of outer membrane permeability mutants in Escherichia coli K-12.

机译：大肠杆菌K-12中外膜渗透突变体的分离与表征。

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Escherichia coli normally requires the lamB gene for the uptake of maltodextrins. We have identified and characterized three independent mutations that allow E. coli to grow on maltodextrin in the absence of a functional lamB gene by allowing maltodextrins with a molecular weight greater than 1,000 to cross the outer membrane barrier. Two of the mutations map to the structural gene for the outer membrane porin OmpF, and the remaining mutation maps to the structural gene for the second major outer membrane porin, OmpC. These mutations increase the permeability of the outer membrane to small hydrophilic substances, antibiotics, and detergents. These mutations alter the electrophoretic mobility of the respective porin proteins.

机译：大肠杆菌通常需要羊肉基因来吸收麦芽糖糊精。我们已经鉴定并表征了三种独立的突变，其允许大肠杆菌在不存在官能羊肉基因的情况下通过允许麦芽糖糊精大于1,000以穿过外膜屏障而在不存在官能羊肉基因上生长。两个突变将映射到外膜孔蛋白OMPF的结构基因，并且剩余的突变映射到第二主要外膜Porin，OMPC的结构基因。这些突变增加了外膜的渗透率，使抗生素和洗涤剂中的小型亲水性物质，抗生素和洗涤剂。这些突变改变了各种孔蛋白的电泳迁移率。

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《Journal of bacteriology》 |1985年第1期|共7页
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S A Benson; A Decloux;
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1. Isolation and characterization of outer membrane permeability mutants in Escherichia coli K-12. [J] . S A Benson, A Decloux Journal of bacteriology . 1985,第1期

机译：大肠杆菌K-12中外膜渗透突变体的分离与表征。
2. Isolation and characterization of outer membrane permeability mutants in Escherichia coli K-12. [J] . S A Benson, A Decloux Journal of bacteriology . 1985,第1期

机译：大肠杆菌K-12中外膜渗透突变体的分离与表征。
3. Synthesis of outer membrane proteins in cpxA cpxB mutants of Escherichia coli K-12. [J] . J McEwen, L Sambucetti, P M Silverman Journal of bacteriology . 1983,第1期

机译：大肠杆菌K-12的cpxA cpxB突变体中外膜蛋白的合成。
4. Effect of silver coated carbon nanotubes on metabolically essential genes expression and outer membrane protein profile of Escherichia coli [C] . Aral A Chaudhari, Shree R. Singh, Shreekumar Pillai Biotech, biomaterials and biomedical . 2016

机译：涂银碳纳米管对大肠杆菌代谢必需基因表达和外膜蛋白谱的影响
5. Lipopolysaccharide assembly in Escherichia coli. Part A: Characterization of the function and biogenesis of the two-protein complex involved in LPS assembly at the outer membrane. Part B: Reconstitution of LPS transport across the periplasm via a trans-envelope protein complex [D] . Chng, Shu Sin 2010

机译：大肠杆菌中的脂多糖组装体。 A部分：表征LPS组装在外膜上的两种蛋白质复合物的功能和生物发生。 B部分：通过跨包膜蛋白复合物重建脂多糖跨周质的运输
6. Isolation and characterization of outer membrane permeability mutants in Escherichia coli K-12. [O] . S A Benson, A Decloux 1985

机译：大肠杆菌K-12中外膜通透性突变体的分离和表征。
7. Isolation and characterization of outer membrane permeability mutants in Escherichia coli K-12 [O] . S A Benson, A Decloux 1985

机译：大肠杆菌K-12中外膜渗透突变体的分离与表征
8. Synthesis of Aerobactin and a 76,000-Dalton Iron-Regulated Outer Membrane Protein by Escherichia coli K-12-Shigella flexneri Hybrids and by Enteroinvasive Strains of Escherichia coli [R] . Griffiths, E., Stevenson, P., Hale, T. L., 1985

机译：大肠埃希氏菌K-12-shigella flexneri杂交种和大肠杆菌肠道侵袭菌合成aerobactin和76,000-Dalton铁调节的外膜蛋白

Isolation and characterization of outer membrane permeability mutants in Escherichia coli K-12.

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