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首页> 外文期刊>Journal of bacteriology >Amino acid transport and metabolism in nitrogen-starved cells of Saccharomyces cerevisiae.
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Amino acid transport and metabolism in nitrogen-starved cells of Saccharomyces cerevisiae.

机译：亚溴菌酿酒酵母氮饥饿细胞中的氨基酸输送和代谢。

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Nitrogen-starved yeast derepress a general amino acid permease which transports basic and hydrophobic amino acids. Although both groups of amino acids are metabolized, the derivatives of the basic amino acids are retained by the cells, whereas those of the hydrophobic amino acids are released as acidic and neutral deaminated derivatives. The release of the deaminated derivatives of the hydrophobic amino acids only occurs in the presence of glucose, which presumably produces amino acceptors. The accumulation of intracellular amino acids results in trans-inhibition of the uptake of exogenous amino acids whether the intracellular amino acid is a basic amino acid or the product of intracellular transamination from a hydrophobic amino acid. Variation of permease and transaminase activity was measured during growth under repressed (ammonia-grown) and derepressed (proline-grown) conditions. Maximum levels for both activities occurs at the mid-exponential phase.
机译：氮饥饿的酵母DEREPRESα透析碱性氨基酸透气，其传输碱性和疏水性氨基酸。尽管代谢两组氨基酸，但碱性氨基酸的衍生物被细胞保留，而疏水性氨基酸的衍生物被释放为酸性和中性脱胺衍生物。疏水性氨基酸的脱胺衍生物的释放仅发生在葡萄糖存在下，这可能产生氨基受体。细胞内氨基酸的积累导致外源氨基酸的摄取的反式抑制，无论是细胞内氨基酸是碱性氨基酸还是来自疏水性氨基酸的细胞内缩进的产物。在抑制（氨生长）和大规模（脯氨酸）条件下的生长期间测量允许释放和转氨酶活性的变异。两种活动的最大水平发生在中指数阶段。

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