• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Journal of Virology >Adenovirus DNA-binding protein in cells infected with wild-type 5 adenovirus and two DNA-minus, temperature-sensitive mutants, H5ts125 and H5ts149.
【24h】

Adenovirus DNA-binding protein in cells infected with wild-type 5 adenovirus and two DNA-minus, temperature-sensitive mutants, H5ts125 and H5ts149.

机译：腺病毒DNA结合蛋白在感染野生型腺病毒和两个DNA-minus，温度敏感突变体，H5TS125和H5TS149的细胞中。

获取原文
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Studies have been done to characterize further H5ts125, an adenovirus type 5 conditionally lethal, temperature-sensitive (ts) mutant defective in initiation of DNA synthesis and to investigate whether the single-strand-specific DNA-binding (72,000 molecular weight) protein is coded by the mutated viral gene. When H5ts125-infected cells were labeled with [35S]methionine at 32 degrees C and then incubated without isotope at 39.5 degrees C, the mutant's nonpermissive temperature, the 72,000 molecular weight polypeptide was progressively degraded. Immunofluorescence examination of cells infected with wild-type virus, H5ts125, and H5ts149 (a second, unique DNA-minus mutant) showed that immunologically reactive DNA-binding protein was barely detectable in H5ts125-infected cells at 39.5 degrees C, whereas this protein was present in wild-type- and H5TS149-infected cells, that the protein made at 32 degrees C in H5ts125-infected cells lost its ability to bind specific DNA-binding protein antibody when the infected cells were shifted to 39.5 degrees C, and that if H5ts125-infected cells were shifted from the restrictive temperature to 32 degrees C, even in the presence of cycloheximide to stop protein synthesis, immunologically reactive DNA-binding protein reappeared.
机译：已经进行了研究以表征另外的H5TS125，腺病毒型5条条件性致命，温度敏感（TS）突变体在开始DNA合成中缺陷，并研究单链特异性DNA结合（72,000分子量）蛋白是否编码通过突变的病毒基因。当在32℃下用[35s]甲硫氨酸标记H5TS125感染的细胞然后在39.5℃下孵育而没有同位素，突变体的非智能温度，72,000分子量多肽逐渐降解。免疫荧光检查用野生型病毒，H5TS125和H5TS149感染的细胞（第二种，独特的DNA-minus突变体）显示，在39.5℃下在H5TS125感染细胞中几乎可以检测免疫活性DNA结合蛋白，而该蛋白质是存在于野生型和H5TS149感染的细胞中，即在H5TS125感染的细胞中在32摄氏度中制备的蛋白质在感染的细胞移至39.5℃时，损失了其结合特异性DNA结合蛋白抗体的能力，如果H5TS125感染的细胞从限制性温度转移到32℃，即使在环己酰亚胺的存在下，也可以停止蛋白质合成，从免疫反应性DNA结合蛋白再现出来。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号