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首页> 外文期刊>Journal of bacteriology >R-plasmid-mediated chromosomal gene transfer in Agrobacterium tumefaciens.
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R-plasmid-mediated chromosomal gene transfer in Agrobacterium tumefaciens.

机译：R-质粒介导的土壤杆菌癌症染色体基因转移。

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Although several techniques are available for transferring the Ti plasmids from one strain of agrobacterium tumefaciens to another, there are no reproducible methods for analysis of chromosomal markers in this phytopathogen. The R plasmid, R68.45, is known to show chromosomal mobilizing ability in several bacterial genera including the closely related Rhizobia. R68.45 was transferred into the prototrophic A. tumefaciens strain 15955. Ten kanamycin-resistant transconjugant clones were tested for chromosomal mobilizing ability by mating with strain SA10, rifampin- and streptomycin-resistant histidine auxotroph of strain 15955. Of the 10 donor clones, 2 showed high chromosomal mobilizing ability. Between 1,000 and 2,000 His+ colony-forming units per ml were obtained, a value 10 to 20 times greater than can be accounted for by spontaneous reversion. Sequential recloning and matings resulted in the isolation of relatively stable donor cultures. Chromosome gene transfer is dependent upon the presence in the donor of R68.45. Donors lacking an R plasmid or harboring the closely related plasmid RP4 failed to yield His+ transconjugants. With strain SA11, a methionine auxotroph of strain SA10, coinheritance of histidine and methionine independence could be demonstrated. Approximately half of the transconjugants also inherited R68.45. These results indicate that A. tumefaciens 15955 is capable of undergoing host chromosomal genetic exchange.
机译：虽然有几种技术可用于将Ti质粒转移到另一种土壤杆菌的菌株，但没有可再现的方法用于分析该植物病变中的染色体标记。已知R质粒R68.45显示出几种细菌属的染色体动员能力，包括密切相关的根瘤菌。将R68.45转移到原型A. Tumefaciens菌株15955中。通过与菌株SA10，利福平和链霉素抗性组胞菌毒素的抑制菌株15955，测试10个Kanamycin抗性的经克隆，用于染色体动员能力。图2显示了高染色体动员能力。获得每ML的1,000至2,000个他+菌落形成单位，其值大于自发逆转的值10至20倍。连续闭合和聚类导致分离相对稳定的供体培养物。染色体基因转移取决于在R68.45供体中的存在。缺乏r质粒或含有密切相关质粒RP4的供体未能产生他的+经+ jugants。对于菌株SA11，可以证明菌株SA10的甲硫氨酸助剂，组氨酸和甲硫氨酸独立的突然植物。大约一半的经历委员还继承了R68.45。这些结果表明A.Tumefaciens 15955能够进行宿主染色体遗传交换。

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