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首页> 外文期刊>Infection and immunity >Demonstration of an iron-siderophore-binding protein in the outer membrane of Pseudomonas aeruginosa.
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Demonstration of an iron-siderophore-binding protein in the outer membrane of Pseudomonas aeruginosa.

机译:铜绿假单胞菌外膜中铁 - 膀胱结合蛋白的示范。

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Outer membranes prepared from Pseudomonas aeruginosa grown in low-iron medium bind three times as much [59Fe]pyochelin as do membranes from cells grown in high-iron medium. The deletion of pyochelin reduced 59Fe binding to background levels. Autoradiographic analysis of sodium dodecyl sulfate polyacrylamide gel electrophoretograms of outer membrane preparations previously incubated with [59Fe]pyochelin revealed that the iron-siderophore complex bound to a low-molecular-weight protein of approximately 14,000.
机译:从低铁介质中生长的假单胞菌铜绿假单胞菌制备的外膜结合了来自高铁介质中生长的细胞的膜的三倍[59Fe] yochelin的三倍。损失肥皂素减少了59Fe与背景水平的结合。外膜制剂的外膜制剂的十二烷基硫酸钠聚丙烯酰胺凝胶电泳图的放射性分析表明,铁 - 膀胱复合物结合到约14,000的低分子量蛋白。

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