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首页> 外文期刊>Molecular and Cellular Biology >Isolation and functional analysis of sporulation-induced transcribed sequences from Saccharomyces cerevisiae.
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Isolation and functional analysis of sporulation-induced transcribed sequences from Saccharomyces cerevisiae.

机译：孢子瘤酿酒酵母孢子诱导的转录序列的分离与功能分析。

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Strains of the yeast Saccharomyces cerevisiae that are heterozygous for the mating-type locus (MATa/MAT alpha) undergo meiosis and spore formation when they are starved for nitrogen and are provided with a nonfermentable carbon source such as potassium acetate. Haploids and diploids homozygous for the mating-type locus (MAT alpha/MAT alpha or MATa/MATa) are asporogenous and undergo neither meiosis nor spore formation when incubated under the same conditions. A small number of genes produce transcripts that appear to be induced specifically in sporulating cells. These transcripts either are not found or are present at much lower levels both in vegetatively growing cells and in cells from asporogenous strains that have been incubated in sporulation medium. Several genes complementary to these MATa/MAT alpha-dependent sporulation-induced transcripts were isolated from a gene-size insert yeast-lambda recombinant DNA library, by differential-plaque filter hybridization. An attempt was made to determine the function of three of these genes by mutating them in the yeast genome with in vitro mutagenesis and one-step gene replacement techniques. One gene was extensively disrupted by both a 0.3-kilobase deletion and the insertion of two large DNA sequences at different sites within the gene. Surprisingly, this compound mutation did not appear to affect meiosis or the production of viable ascospores, indicating that this gene was dispensable for differentiation. The other two genes were disrupted by simple insertion mutations at a site where it was possible that they might still possess some gene activity. These mutations also did not appear to affect sporulation. These results suggest that not all sporulation-induced genes are essential for meiosis and the production of viable ascospores under the conditions examined.
机译：酵母酿酒酵母的菌株，其对交配型基因座（Mata / Mata / Mata / Mata / Mata / Mata / Mata / Mata / Mata / Mata / Mata alpha）的菌株在缺乏氮气时接受过分裂和孢子形成，并且具有乙酸钾的非可变碳源如乙酸钾。单倍体和交配型基因座（Matα/Matα或Mata / Mata）纯合的单倍体和二倍体是生殖的，并且在相同条件下孵育时既不均未经历过减数分裂，也没有孢子形成。少量基因产生似乎在孢子形细胞中特别诱导的转录物。这些转录物的未发现或在营养生长细胞和从已经在孢子介质中孵育的生孢菌菌株中的细胞中存在下层的水平低得多。通过差分牙菌滤网杂交，从基因大小插入酵母 - λ重组DNA文库中分离出与这些Mata / solα-依赖性孢子化诱导的转录物互补的几个基因。通过在酵母基因组中突变具有体外诱变和一步基因替代技术，通过在酵母基因组中突变它们来确定三种这些基因的功能。通过0.3千碱基缺失和在基因内的不同位点插入两个大DNA序列的一个基因广泛破坏。令人惊讶的是，这种复合突变似乎没有影响减数分裂或生产可行性囊孢子，表明该基因是分化的分化。另外两个基因被简单的插入突变中断在遗址上，它们可能仍然具有一些基因活性。这些突变也没有似乎影响孢子。这些结果表明，并非所有的孢子素诱导的基因对于减数分裂是必不可少的，并且在所检查条件下生产可行性囊孢子。

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