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首页> 外文期刊>Journal of environmental science and health >Evaluation of the impact of matrix effects in LC/MS measurement on the accurate quantification of neonicotinoid pesticides in food by isotope-dilution mass spectrometry
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Evaluation of the impact of matrix effects in LC/MS measurement on the accurate quantification of neonicotinoid pesticides in food by isotope-dilution mass spectrometry

机译：同位素稀释质谱法评估LC / MS测量中基质效应对食品中新烟碱类农药准确定量的影响

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摘要

The use of isotope-labeled internal standards is the most widely accepted approach to overcome the matrix effects on quantification of pesticides in food by LC/MS. We evaluated the impact of the matrix effects on quantification of six neonicotinoid pesticides, acetamiprid, clothianidin, dinotefuran, imidacloprid, thiacloprid, and thiamethoxam, in food by using deuterated internal standards. The calibration curves for each pesticide were obtained by using matrix-free and matrix-matched calibration solutions with blank brown rice, carrot, and green onion extracts. For brown rice and carrot, the matrix effects were not observed. In contrast, the slopes of calibration curves for each pesticide were influenced by presence of green onion extracts in calibration solutions (variability of the slopes was 4-9%), because the ratios of peak area for native pesticide to those for internal standards were influenced by matrix. The spike-and-recovery test with green onion was also performed. The analytical values obtained by using matrix-free calibration solution were biased from the spiked concentration, whereas those obtained by using matrix-matched calibration solution were comparable to the spiked concentration. These results indicate that matrix-matched calibration solution should be used for accurate quantification of neonicotinoid pesticides in food by LC/MS using deuterated internal standards.
机译：同位素标记的内标的使用是克服LC / MS对食品中农药定量中基质影响的最广泛接受的方法。我们使用氘化内标评估了基质效应对食品中六种新烟碱类农药（乙酰胺类，可比丁类，二甲呋喃，吡虫啉，噻虫啉和噻虫嗪）的定量影响。通过使用无基质和基质匹配的校准溶液以及空白糙米，胡萝卜和葱提取物获得每种农药的校准曲线。对于糙米和胡萝卜，未观察到基质效应。相反，每种农药的校准曲线的斜率受校准溶液中葱提取物的存在的影响（斜率的变化为4-9％），因为天然农药与内标的峰面积之比受到影响按矩阵。还进行了葱的加标回收试验。使用无基质校准溶液获得的分析值与加标浓度有偏差，而使用基质匹配校准溶液获得的分析值与加标浓度相当。这些结果表明，应使用基质匹配的校准溶液通过氘代内标通过LC / MS对食品中的新烟碱类农药进行准确定量。

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