...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Journal of environmental science and health >Characterization of bacterial diversity in an atrazine degrading enrichment culture and degradation of atrazine, cyanuric acid and biuret in industrial wastewater
【24h】

Characterization of bacterial diversity in an atrazine degrading enrichment culture and degradation of atrazine, cyanuric acid and biuret in industrial wastewater

机译：阿特拉津降解富集培养中细菌多样性的表征以及工业废水中阿特拉津，氰尿酸和缩二脲的降解

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

An enrichment culture was used to study atrazine degradation in mineral salt medium (MSM) (T1), MSM+soil extract (1:1, v/v) (T2) and soil extract (T3). Results suggested that enrichment culture required soil extract to degrade atrazine, as after second sequential transfer only partial atrazine degradation was observed in T1 treatment while atrazine was completely degraded in T2 and T3 treatments even after fourth transfer. Culture independent polymerase chain reaction-denaturing gradient gel electrophoresis (PCR-DGGE) technique confirmed selective enrichment of genus Bacillus along with Pseudomonas and Burkholderia. Degradation of atrazine/metabolites in the industrial wastewater was studied at different initial concentrations of the contaminants [wastewater-water (v/v) ratio: T1, 1:9; T2, 2:8; T3, 3:7; T4, 5:5 and T5, undiluted effluent]. The initial concentrations of atrazine, cyanuric acid and biuret ranged between 5.32 and 53.92 μg mL~(-1), 265.6 and 1805.2 μg mL~(-1) and 1.85 and 16.12 μg mL~(-1), respectively. The enrichment culture was able to completely degrade atrazine, cyanuric acid and biuret up to T4 treatment, while no appreciable degradation of contaminants was observed in the undiluted effluent (T5). Inability of enrichment culture to degrade atrazine/metabolites might be due to high concentrations of cyanuric acid. Therefore, a separate study on cyanuric acid degradation suggested: (ⅰ) no appreciable cyanuric acid degradation with accumulation of an unidentified metabolite in the medium where cyanuric acid was supplemented as the sole source of carbon and nitrogen; (ⅱ) partial cyanuric acid degradation with accumulation of unidentified metabolite in the medium containing additional nitrogen source; and (ⅲ) complete cyanuric acid degradation in the medium supplemented with an additional carbon source. This unidentified metabolite observed during cyanuric acid degradation and also detected in the enrichment culture inoculated wastewater samples, however, was degraded up to T4 treatments and was persistent in the T5 treatment. Probably, accumulation of this metabolite inhibited atrazine/cyanuric acid degradation by the enrichment culture in undiluted wastewater.
机译：富集培养用于研究at去津在矿物盐培养基（MSM）（T1），MSM +土壤提取物（1：1，v / v）（T2）和土壤提取物（T3）中的降解。结果表明，富集培养需要土壤提取物降解阿特拉津，因为在第二次连续转移后，在T1处理中仅观察到部分阿特拉津降解，而即使在第四次转移后，A2在T2和T3处理中也被完全降解。不依赖培养物的聚合酶链反应-变性梯度凝胶电泳（PCR-DGGE）技术证实了芽孢杆菌属以及假单胞菌和伯克霍尔德氏菌的选择性富集。研究了在不同初始污染物浓度下废水中r去津/代谢物的降解情况[废水与水（v / v）的比例：T1，1：9； T2，2：8; T3，3：7; T4、5：5和T5，未稀释的废水]。 r去津，氰尿酸和缩二脲的初始浓度分别在5.32和53.92μgmL〜（-1），265.6和1805.2μgmL〜（-1）和1.85和16.12μgmL〜（-1）之间。浓缩培养物能够完全降解阿特拉津，氰尿酸和缩二脲直至T4处理，而未稀释废水（T5）中未观察到明显的污染物降解。富集培养无法降解阿特拉津/代谢物可能是由于高浓度的氰尿酸。因此，另一项有关氰尿酸降解的研究表明：（ⅰ）在补充了氰尿酸作为唯一碳和氮源的培养基中，未发现未知代谢产物的积累，没有明显的氰尿酸降解； （ⅱ）在含有额外氮源的培养基中，氰尿酸部分降解，伴随着未知代谢产物的积累； （ⅲ）在添加了额外碳源的培养基中，氰尿酸完全降解。在氰尿酸降解期间观察到的这种不确定的代谢物，在富集培养接种的废水样品中也检测到，但是在T4处理之前降解，并在T5处理中持续存在。通过在未稀释废水中进行富集培养，这种代谢产物的积累可能会抑制阿特拉津/氰尿酸的降解。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号