• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Journal of environmental science and health >PCR-RAPD profiling of Fusarium spp. causing guava wilt disease in India
【24h】

PCR-RAPD profiling of Fusarium spp. causing guava wilt disease in India

机译：镰刀菌的PCR-RAPD分析。在印度造成番石榴枯萎病

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Wilt is a serious disease of the guava crop in India. Fusarium oxysporum f. sp. psidii and F. solani have been reported as causative agents of this disease. In this study, 42 isolates each of F. oxysporum f. sp. psidii and F. solani, were isolated from guava cultivars and characterized by randomly amplified polymorphic DNA (RAPD) method. Thirty RAPD primers were tested in the genome of Fusarium spp. and the number of scorable bands for corresponding primer ranged from 1 -8 with an average of 5 bands per individual. DNA band size ranged from 200 bp to 5090 bp. A 0.21 per cent polymorphism was found in individual isolates of F. solani indicating that the 42 isolates were similar. However, a 2.58 percent polymorphism among individual isolates of F. oxysporum f.sp. psidii showed a higher level of genetic diversity. Cluster analysis of the RAPD band patterns clearly separated the isolates of F. oxysporum f.sp. psidii into three clusters. Two clusters were formed with F. solani isolates, showing a higher degree of similarity. Unique fingerprint profiles generated by the PCR-RAPD can be exploited for genetic characterization purposes.
机译：枯萎病是印度番石榴的一种严重病。尖孢镰刀菌f。 sp。 psidii和F. solani被报道为该病的病原体。在这项研究中，有42株分离到了F. oxysporum f。 sp。从番石榴品种中分离了psidii和sol。F. solani，并通过随机扩增多态性DNA（RAPD）方法对其进行了表征。在镰刀菌属的基因组中测试了30种RAPD引物。相应引物的可计谱带数范围为1 -8，每人平均5条谱带。 DNA条带大小介于200 bp至5090 bp之间。在sol。F. solani的各个分离物中发现0.21％的多态性，表明42个分离物相似。然而，在F.oxysporum f.sp.的单个分离株之间有2.58％的多态性。 psidii显示出更高水平的遗传多样性。 RAPD条带模式的聚类分析清楚地分离了尖孢镰刀菌f.sp. psidii分为三个簇。与茄形镰刀菌分离物形成两个簇，显示出更高的相似度。 PCR-RAPD产生的独特指纹图谱可用于遗传表征。

著录项

  • 来源
    《Journal of environmental science and health》 |2012年第4期|p.315-325|共11页
  • 作者

    V. K. GUPTA;

  • 作者单位

    National University of Ireland, Galway, Ireland,Department of Biotechnology, MITS University, Rajasthan, India Molecular Glycobiotechnology Unit, Department of Biochemistry, School of Natural Sciences, National University of Ireland, Galway, Ireland;

  • 收录信息
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

    PCR-RAPD; F. oxysporum f. sp. psidii; F. solani; guava wilt;

    机译：PCR-RAPD;F.oxysporum f。 sp。 psidii;F. solani;番石榴;

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号