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In vitro changes in porcine ovarian granulosa cells induced by copper

机译：铜诱导的猪卵巢颗粒细胞的体外变化

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摘要

Objective of this in vitro study was to examine the secretion activity (progesterone and insulin-like growth factor Ⅰ) of porcine ovarian granulosa cells after copper (Cu) addition and to outline a potential intracellular mediator (cyclin B1) of its effects. It also aimed at investigating the apoptotic potential of Cu on porcine ovarian granulosa cells after addition in vitro. Ovarian granulosa cells were incubated with copper sulphate (CuSO_4·5H_2O) at the doses 0.33,0.40,0.50,1.0 and 2.0 μL mL~(-1) for 18 h and compared with control group without Cu addition. Release of progesterone (P_4) and insulin-like growth factor Ⅰ (IGF-I) by granulosa cells was assessed by RIA, expression of cyclin B1 by immunocytochemistry and apoptosis by TUNEL assay. Observations show that P_4 release by granulosa cells was inhibited while the release of IGF-I and cyclin B1 was stimulated significantly (P ＜ 0.05) by CuSO_4·5H_2O addition at the dose 2.0 μL mL~(-1). Also, addition of CuSO_4·5H_2O at the lowest dose used in the study (0.33 μL mL~(-1)) significantly (P ＜ 0.05) decreased apoptosis in granulosa cells. In conclusion, results indicate dose dependent effect of Cu on (1) secretion of steroid hormone progesterone and growth factor IGF-I, (2) expression of cyclin B1 as marker of proliferation of porcine ovarian granulosa cells, (3) apoptosis of porcine ovarian granulosa cells and, (4) that the effect of Cu on ovarian cell proliferation could be mediated by IGF-I and cyclin B1. Obtained data suggest interference of Cu in the pathways of proliferation of porcine ovarian granulosa cells through hormonal and intracellular peptide cyclin B1.
机译：这项体外研究的目的是检查添加铜（Cu）后猪卵巢颗粒细胞的分泌活性（孕酮和胰岛素样生长因子Ⅰ），并概述其潜在的细胞内介质（细胞周期蛋白B1）。它还旨在研究铜在体外添加后对猪卵巢颗粒细胞的凋亡潜力。将卵巢颗粒细胞与硫酸铜（CuSO_4·5H_2O）分别以0.33、0.40、0.50、1.0和2.0μLmL〜（-1）的剂量孵育18 h，并与未添加Cu的对照组进行比较。用RIA法测定颗粒细胞释放孕酮（P_4）和类胰岛素生长因子Ⅰ（IGF-I）的量，用免疫细胞化学法检测细胞周期蛋白B1的表达，用TUNEL法测定细胞凋亡。观察结果表明，添加CuSO_4·5H_2O剂量为2.0μLmL〜（-1）时，颗粒细胞释放的P_4受到抑制，而IGF-I和细胞周期蛋白B1的释放受到显着刺激（P ＜0.05）。另外，以研究中使用的最低剂量（0.33μLmL〜（-1））添加CuSO_4·5H_2O显着（P ＜0.05）降低了颗粒细胞的凋亡。总之，结果表明Cu对（1）甾体激素孕酮和生长因子IGF-I的分泌具有剂量依赖性，（2）细胞周期蛋白B1的表达是猪卵巢颗粒细胞增殖的标志，（3）猪卵巢细胞凋亡（4）铜对卵巢细胞增殖的影响可能是由IGF-I和细胞周期蛋白B1介导的。获得的数据表明，铜通过激素和细胞内肽细胞周期蛋白B1干扰了猪卵巢颗粒细胞的增殖途径。

著录项

  • 来源
    《Journal of Environmental Science and Health》 |2014年第6期|625-633|共9页
  • 作者

    SHUBHADEEP ROYCHOUDHURY; JOZEF BULLA; ALEXANDER V. SIROTKIN; ADRIANA KOLESAROVA;

  • 作者单位

    Department of Life Science & Bioinformatics, Assam University, Silchar, India;

    Department of Animal Physiology, Faculty of Biotechnology and Food Sciences, Slovak University of Agriculture, Nitra, Slovak Republic;

    Department of Genetics and Reproduction, Animal Production Research Centre Nitra, Luzianky, Slovak Republic;

    Department of Animal Physiology, Faculty of Biotechnology and Food Sciences, Slovak University of Agriculture, Nitra, Slovak Republic;

  • 收录信息 美国《科学引文索引》(SCI);美国《工程索引》(EI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

    Copper; porcine; ovarian granulosa cells; secretion activity;

    机译：铜;猪卵巢颗粒细胞分泌活动;

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