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首页> 外文期刊>Journal of Environmental Science and Health >Physiological aspects of the interaction of multi-walled carbon nanotubes with Beauveria bassiana (Balsamo) Vuillem in entomopathogenic spores
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Physiological aspects of the interaction of multi-walled carbon nanotubes with Beauveria bassiana (Balsamo) Vuillem in entomopathogenic spores

机译：昆虫致病孢子中多壁碳纳米管与球孢白僵菌（Balveria bassiana（Balsamo）Vuillem）相互作用的生理方面

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摘要

The objective of the study was to evaluate the in vitro effect of aqueous suspensions of commercial multi-walled carbon nanotubes (MWCNTs) on spores of three entomopathogenic fungus species of Beauveria bassiana. Spores were placed in contact with MWCNTs for 1, 24, 72 and 216 h and then an in vitro evaluation in the subculture was performed on the linear growth of the vegetative mycelium of the spores after the contact with nanomaterial, as well as its sporulation after completion of the culture. No fungistatic effect of MWCNTs against B. bassiana spores was identified. Modification of vegetative mycelium surface growth in the culture of spores after contact with MWCNTs was mainly due to less effective inoculation, especially when shorter contact times with nanomaterial were applied. For one of the investigated strains, growth stimulation was observed in the culture of spores in contact with nanotubes for 72 and 216 h. Based on the significant intensification of sporulation observed in combination with the longest (216 h) contact with the investigated spore strains before culture with MWCNTs, it can be concluded that the tested nanomaterial constitutes a stress factor and may modify the metabolism of B. bassiana cells. Detailed evaluation of B. bassiana metabolic changes induced by MWCNTs should be conducted.
机译：这项研究的目的是评估商业多壁碳纳米管（MWCNTs）的水悬浮液对三种球孢白僵菌病原真菌的孢子的体外作用。将孢子与MWCNT接触1、24、72和216 h，然后在传代培养中进行体外评估，以评估与纳米材料接触后孢子的营养菌丝体的线性生长及其在形成后的孢子形成文化的完成。没有发现MWCNTs对球孢杆菌孢子的抑菌作用。与MWCNTs接触后，孢子培养中营养菌丝体表面生长的改变主要是由于接种效果较差，尤其是当应用与纳米材料的接触时间较短时。对于所研究的菌株之一，在与纳米管接触72和216小时的孢子培养物中观察到生长刺激。基于观察到的孢子形成显着增强以及与所研究的孢子菌株接触时间最长（216 h）的接触，然后用MWCNTs培养，可以得出结论，所测试的纳米材料构成了应激因子，并可能改变了球孢白僵菌细胞的代谢。应进行由碳纳米管诱导的球孢杆菌代谢变化的详细评估。

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