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首页> 外文期刊>Journal of Environmental Science and Health >Analysis of aryl hydrocarbon receptor ligands in kraft mill effluents by a combination of yeast bioassays and CG-MS chemical determinations
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Analysis of aryl hydrocarbon receptor ligands in kraft mill effluents by a combination of yeast bioassays and CG-MS chemical determinations

机译：结合酵母生物测定法和CG-MS化学测定法分析牛皮纸厂废水中的芳烃受体配体

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摘要

Aryl Hydrocarbon Receptor (AhR) ligands also known as dioxin-like compounds, constitute a substantial part of the total toxicity from many pollution sources, including pulp mill effluents. The aim of this article was to evaluate dioxin-like activity in different kraft mill effluents by a combination of yeast bioassays and gas chromatography-mass spectrometry (GC-MS) chemical analysis. The study includes kraft mill effluents from three sources of raw material: Pinus radiata, Eucalyptus globulus and a combination of both (50% each). The Recombinant Yeast Assay (RYA) showed an effective concentration of AhR ligands more than 30-fold higher in Eucalyptus globulus than in Pinus radiata effluents. Our results suggest that specific ligands, rather than the total amount of extractive material, determined the observed activity. Analysis of extract composition by GC-MS indicated that moderately hydrophobic aromatic compounds were likely responsible for the observed dioxin-like activity. In particular, benzaldehyde derivatives appeared as candidates for eliciting the observed dioxin-like activity in pulp mill effluents, giving their structural properties and their high concentration in AhR ligand-rich samples.
机译：芳基碳氢化合物受体（AhR）配体也称为二恶英样化合物，构成了包括制浆厂废水在内的许多污染源所产生的全部毒性的很大一部分。本文的目的是通过酵母生物测定和气相色谱-质谱（GC-MS）化学分析相结合的方法，评估不同牛皮纸厂废水中的二恶英样活性。该研究包括来自三种原材料的牛皮纸厂废水：辐射松，桉树球和两者的混合物（各占50％）。重组酵母分析（RYA）显示，在桉树中的有效AhR配体浓度比在辐射松中的有效浓度高30倍以上。我们的结果表明，特定的配体而不是萃取材料的总量决定了观察到的活性。通过GC-MS分析提取物组成表明，中等疏水性芳香族化合物可能是所观察到的二恶英样活性的原因。尤其是，苯甲醛衍生物似乎是在纸浆厂废水中引起所观察到的二恶英类活性的候选物，从而使其结构特性和它们在富含AhR配体的样品中的浓度很高。

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  • 来源
    《Journal of Environmental Science and Health》 |2013年第2期|145-151|共7页
  • 作者

    SOLEDAD CHAMORRO; ELIZABETH MONSALVEZ; BENJAMIN PINA; ALBA OLIVARES; VICTOR HERNANDEZ; JOSE BECERRA; GLADYS VIDAL;

  • 作者单位

    Environmental Science Center EULA-Chile, University of Conception, Conception, Chile;

    Environmental Science Center EULA-Chile, University of Conception, Conception, Chile;

    Institute of Environmental Assessment and Water Research (IDAEA-CSIC), Barcelona, Spain;

    Institute of Environmental Assessment and Water Research (IDAEA-CSIC), Barcelona, Spain;

    Natural Products Chemistry Laboratory, Natural and Oceanographic Science, University of Conception, Conception, Chile;

    Natural Products Chemistry Laboratory, Natural and Oceanographic Science, University of Conception, Conception, Chile;

    Environmental Science Center EULA-Chile, University of Conception, P.O. Box 160-C, Conception, Chile;

  • 收录信息 美国《科学引文索引》(SCI);美国《工程索引》(EI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

    Pulp mill effluents; recombinant yeast assay; dioxin-like; compounds; GC-MS; ECF kraft pulp;

    机译：纸浆厂废水;重组酵母检测类二恶英化合物;气相色谱-质谱ECF牛皮纸浆;

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