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首页> 外文期刊>Journal of Environmental Science and Health >Multi-level stressor analysis from the DNA/biochemical level to community levels in an urban stream and integrative health response (IHR) assessments
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Multi-level stressor analysis from the DNA/biochemical level to community levels in an urban stream and integrative health response (IHR) assessments

机译：从城市中的DNA /生化水平到社区水平的多级应激源分析和综合健康响应（IHR）评估

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摘要

The objectives of this study were to identify multi-level stressors at the DNA/biochemical level to the community level in fish in an urban stream and to develop an integrative health response (IHR) model for ecological health diagnosis. A pristine control site (S_c) and an impacted site (S_i) were selected from among seven pre-screened sites studied over seven years. Various chemical analyses indicated that nutrient enrichment (Nitrogen, Phosphorus) and organic pollution were significantly greater (t＞8.783, p＜0.01) at the S_i site compared to the S_c site. Single-cell gel electrophoresis (comet assays) of DNA-level impairment indicated significantly (t = 5.678, p＜0.01) greater tail intensity, expressed as % tail-DNA, at the S_i site and genotoxic responses were detected in the downstream reach. Ethoxyresorufin-O-deethylase (EROD) assays, as a physiological bioindicator, were 2.8-fold higher (p＜0.05, NK-test after ANOVA) at the S_i site. Tissue analysis using a necropsy-based health assessment index (NHAI) showed distinct internal organ disorders in three tissues, i.e., liver, kidney, and gill, at the S_i site. Population-level analysis using the sentinel species Zacco platypus showed that the regression coefficient (b) was 3.012 for the S_i site and 2.915 for the S_c site, indicating population skewness in the downstream reach. Community-level health was impaired at the S_i site based on an index of biological integrity (IBI), and physical habitat modifications were identified by a qualitative habitat evaluation index (QHEI). Overall, the model values for the integrative health response (IHR), developed using the star plot approach, were 3.22 (80.5%) at the S_c site and 0.74 (18.5%) at the S_i site, indicating that, overall, ecological health impairments were evident in the urban reach. Our study was based on multi-level approaches using biological organization and the results suggest that there is a pivotal point of linkage between mechanistic understanding and real ecological consequences of environmental stressors.
机译：这项研究的目的是在城市河流中的鱼类中，从DNA /生化水平到社区水平识别多级应激源，并开发用于生态健康诊断的综合健康响应（IHR）模型。从经过七年研究的七个预先筛选的站点中选择了一个原始的控制站点（S_c）和一个受影响的站点（S_i）。各种化学分析表明，与S_c位点相比，S_i位点的养分富集（氮，磷）和有机污染显着更大（t＞ 8.783，p ＜0.01）。 DNA水平损伤的单细胞凝胶电泳（彗星分析）表明，S_i位点的尾巴强度显着提高（t = 5.678，p ＜0.01），以尾巴DNA表示（％），并且在下游区域检测到了遗传毒性反应。作为生理生物指示剂的乙氧基间苯二酚-O-脱乙基酶（EROD）测定在S_i位点高2.8倍（p ＜0.05，ANOVA后的NK测试）。使用基于尸检的健康评估指数（NHAI）进行的组织分析显示，在S_i部位的三个组织（即肝，肾和g）中存在明显的内部器官疾病。使用前哨物种Zacco platypus的种群水平分析显示，S_i站点的回归系数（b）为3.012，S_c站点的回归系数（b）为2.915，表明下游河段的人口偏斜。基于生物完整性指数（IBI），S_i站点的社区卫生水平受到损害，并且通过定性的栖息地评估指数（QHEI）确定了物理栖息地的改变。总体而言，采用星图法开发的综合健康响应（IHR）模型值在S_c站点为3.22（80.5％），在S_i站点为0.74（18.5％），表明总体上生态健康受损在城市范围内很明显。我们的研究基于使用生物组织的多层次方法，结果表明，在机械理解与环境压力源的实际生态后果之间存在关键的联系点。

著录项

  • 来源
    《Journal of Environmental Science and Health》 |2013年第2期|211-222|共12页
  • 作者

    JAE HOON LEE; JOON HA KIM; HEE-MOCK OH; KWANG-GUK AN;

  • 作者单位

    Department of Biological Science, College of Biological Sciences and Biotechnology, Chungnam National University, Daejeon, Korea;

    Department of Environmental Science and Engineering, Gwangju Institute of Science and Technology, Gwangju, Korea;

    Environmental Biotechnology Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB), Daejeon, Korea;

    Department of Biological Science, College of Biological Sciences and Biotechnology, Chungnam National University, Daejeon, South Korea;

  • 收录信息 美国《科学引文索引》(SCI);美国《工程索引》(EI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

    Integrative health; stressor; genotoxic response; biological integrity; casual assessment; multimetric index;

    机译：综合健康;压力源遗传毒性反应;生物完整性临时评估;多指标索引;

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