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The optimization of biohydrogen production by bacteria using residual glycerol from biodiesel synthesis

机译:利用生物柴油合成过程中残留的甘油优化细菌生产生物氢的能力

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摘要

In this research the production of hydrogen by Klebsiella pneumoniae BLbOl using residual glycerol discharged from a biodiesel fuel production plant was investigated. Klebsiella pneumoniae BLbOl was isolated from a bacteria-rich sludge of an upflow anaerobic sludge blanket reactor (UASB) of a soybean processing plant. A Plackett-Burman design (P-B) and Response Surface Methodology (RSM) were employed to determine the optimal condition for enhanced hydrogen production. The maximal hydrogen production, which was 45.0 mol % and with 98% of glycerol degradation, was achieved with the optimized medium with the following composition: 30 g L~(-1) glycerol; 3 g L~(-1) yeast ex tract 3 g L~(-1) K_2HPO_4; 1 g L~(-1) KH_2PO_4; temperature 39℃ and pH 9.0. These results show the ability of this new strain of effectively converting residual glycerol into value-added energy products.
机译:在这项研究中,研究了肺炎克雷伯氏菌BLbOl使用从生物柴油燃料生产厂排出的残留甘油生产氢的方法。从大豆加工厂的上流厌氧污泥床反应器(UASB)的富细菌污泥中分离出肺炎克雷伯菌。采用Plackett-Burman设计(P-B)和响应表面方法(RSM)确定增强氢气产生的最佳条件。用具有以下组成的优化培养基可达到最大产氢量:45.0 mol%,甘油降解98%。 3 g L〜(-1)酵母提取物3 g L〜(-1)K_2HPO_4; 1克L〜(-1)KH_2PO_4;温度39℃,pH 9.0。这些结果表明,这种新菌株能够有效地将残留的甘油转化为增值的能源产品。

著录项

  • 来源
    《Journal of Environmental Science and Health》 |2011年第13期|p.1461-1468|共8页
  • 作者单位

    Department of Chemistry, Universidade Federal do Rio Grande do Sul, Brazil;

    Biotechnology & Biochemical Engineering Laboratory (BiotecLab), Universidade Federal do Rio Grande do Sul, Brazil;

    Biotechnology & Biochemical Engineering Laboratory (BiotecLab), Universidade Federal do Rio Grande do Sul, Brazil;

    Department of Chemistry, Universidade Federal do Rio Grande do Sul, Brazil;

    Department of Chemistry, Universidade Federal do Rio Grande do Sul, Brazil;

    Department of Chemistry, Universidade Federal do Rio Grande do Sul, Brazil,Instituto de Quimica - UFRGS, Av. Bento Goncalves 9500 - Campus do Vale, 91501-970-Porto Alegre, RS, Brazil;

    Biotechnology & Biochemical Engineering Laboratory (BiotecLab), Universidade Federal do Rio Grande do Sul, Brazil;

    Biotechnology & Biochemical Engineering Laboratory (BiotecLab), Universidade Federal do Rio Grande do Sul, Brazil;

  • 收录信息 美国《科学引文索引》(SCI);美国《工程索引》(EI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

    hydrogen production; biodiesel; residual glycerol; klebsiella pneumoniae;

    机译:制氢生物柴油残留甘油;肺炎克雷伯菌;

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