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首页> 外文期刊>Journal of Environmental Science and Health >Uptake and biochemical response to B[a]P in the sea anemone Anthopleura elegantissima
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Uptake and biochemical response to B[a]P in the sea anemone Anthopleura elegantissima

机译：海葵Anthopleura Elegantissima对B [a] P的吸收及其生化响应

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摘要

In order to evaluate the effects of benzo[a]pyrene (B[a]P), a polycyclic aromatic hydrocarbon (PAH) in the sea anemone Anthopleura elegantissima at the biochemical level. NADPH cytochrome P450 reductase and cytochrome P450 were assayed in A. elegantissima under toxicant. One toxicity test was performed with 75 organisms distributed in 5 groups. Animals in groups G1, G2 and G3 were given increasing B[a]P. Two groups named GC and GS were used as controls. GC was treated with seawater and GS was treated with acetone. After 72 h of exposure, enzymatic activities were determined. Microsomes were isolated from the columnar tissue and exposed in vitro to the toxicant in order to explore their ability to incorporate B[a]P. Basal activity for this enzyme was 1.69 ± 0.18 (Mean ± standard deviation) nmol cyt C red min~(-1) mg~(-1) and there was no significant effect in GS organisms compared to GC organisms. Significant increases were observed in NADPH cytochrome P450 reductase in G3 organisms. In this group, the enzyme activity was 3.53 ± 0.40 nmol cyt C red min~(-1) mg~(-1). For cytochrome P450 content, a gradual increase was observed in organisms in groups Gl to G3. Basal content was 10.25 ± 0.49 pmol mg~(-1) microsomal protein. For G3 animals, P450 content was 27.51 ± 0.32 pmol mg~(-1) microsomal. For the test in vitro, it was found that microsomes isolated from G2 and G3 had the capacity to incorporate this substance when exposed to B[a]P at a level of 4 μM in the surrounding medium. Spectrum recorded from 350 to 450 rim after a 40-min exposure for these groups showed significant difference from spectra obtained for microsomes in GC, GS and G1. It was concluded that the capacity to increase NADPH cytochrome P450 reductase activity as well as to increase NADPH cytochrome P450 reductase activity as well as to increase P450 content shows the ability of A. elegantissima to induce a mixed function oxidase activity in the presence of B[a]P.
机译：为了评估苯并[a] py（B [a] P）的作用，在生化水平上，多环芳烃（PAH）在海葵Anthopleura Elegantissima中的作用。在有毒动物中，在elegant草中测定了NADPH细胞色素P450还原酶和细胞色素P450。对分布在5组中的75种生物体进行了一项毒性试验。 G1，G2和G3组的动物的B [a] P增加。名为GC和GS的两组用作对照。 GC用海水处理，GS用丙酮处理。暴露72小时后，测定酶活性。从柱状组织中分离出微粒体，并将其体外暴露于有毒物质中，以探索其掺入B [a] P的能力。该酶的基础活性为1.69±0.18（平均值±标准偏差）nmol cyt C red min〜（-1）mg〜（-1），与GC生物体相比，GS生物体无显着影响。在G3生物体中观察到NADPH细胞色素P450还原酶显着增加。在该组中，酶活性为3.53±0.40nmol cyt C red min〜（-1）mg〜（-1）。对于细胞色素P450含量，在G1至G3组的生物体中观察到逐渐增加。基础含量为10.25±0.49pmol mg〜（-1）微粒体蛋白。对于G3动物，P450含量为27.51±0.32 pmol mg〜（-1）微粒体。对于体外测试，发现从G2和G3分离的微粒体在周围培养基中以4μM的水平暴露于B [a] P时具有掺入该物质的能力。这些组暴露40分钟后，从350到450边缘记录的光谱显示与从GC，GS和G1中的微粒体获得的光谱显着不同。结论是，增加NADPH细胞色素P450还原酶活性以及增加NADPH细胞色素P450还原酶活性以及增加P450含量的能力表明，在有B []存在的情况下，淡色假单胞菌具有诱导混合功能氧化酶活性的能力。 a] P。

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