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首页> 外文期刊>Marine genomics >Transcriptome sequencing and characterization of Symbiodinium muscatinei and Elliptochloris marina, symbionts found within the aggregating sea anemone Anthopleura elegantissima
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Transcriptome sequencing and characterization of Symbiodinium muscatinei and Elliptochloris marina, symbionts found within the aggregating sea anemone Anthopleura elegantissima

机译:Symbiodinium muscatinei和Elliptochloris marina的转录体测序和表征,聚集在聚集海葵Anthopleura优雅

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There is a growing body of literature using transcriptomic data to study how tropical cnidarians and their photosynthetic endosymbionts respond to environmental stressors and participate in metabolic exchange. Despite these efforts, our understanding of how essential genes function to facilitate symbiosis establishment and maintenance remains limited. The inclusion of taxonomically and ecologically diverse endosymbionts will enhance our understanding of these interactions. Here we characterize the transcriptomes of two very different symbionts found within the temperate sea anemone Anthopleura elegantissima: the chlorophyte Elliptochloris marina and the dinoflagellate Symbiodinium muscatinei. We use a multi-level approach to assess the diversity of genes found across S. muscatinei and E. marina transcriptomes, and compare their overall protein domains with other dinoflagellates and chlorophytes. Our analysis identified several genes that are potentially involved in mitigating stress response (e.g., heat shock proteins pathways for mediating reactive oxygen species) and metabolic exchange (e.g., ion transporters). Finally, we show that S. muscatinei and other Symbiodinium strains are equipped with a high salt peridinin-chl-protein (HSPCP) gene previously identified only in free-living dinoflagellates. The addition of these transcriptomes to the cnidarian-symbiont molecular toolkit will aid in understanding how these vitally important symbiotic relationships are established and maintained across a variety of environmental conditions.
机译:使用转录组数据有一种不断增长的文献,以研究热带的Cnivarians和它们的光合型内膜如何应对环境压力源并参与代谢交换。尽管有这些努力,但我们了解基本基因的作用如何促进共生建立和维护仍然有限。纳入分类学和生态多样化的内糖尿布将提高我们对这些相互作用的理解。在这里,我们的特征在于在温带海葵中发现的两个非常不同的共生的转录om Anthopleura优雅:叶绿素椭圆形玛丽娜和Dinoflagellate Symbiodininium Muscatinei。我们使用多级方法来评估S. Muscatinei和E.Marina转录om在S. Muscatinei和E.Marina转录om的基因的多样性,并将其整体蛋白质结构域与其他Dinoflagellate和叶绿素进行比较。我们的分析鉴定了几种可能参与减轻减轻应力反应的基因(例如,用于介导反应性氧物质的热休克蛋白途径)和代谢交换(例如,离子转运蛋白)。最后,我们表明S. Muscatinei和其他Symbiodinium菌株配备了先前仅在自由终止的DinoflaGates中鉴定的高盐白翅蛋白-CHL-蛋白(HSPCP)基因。将这些转录om对Cnidarian-Symbiont分子工具包提供有助于了解这些重要的重要共生关系如何在各种环境条件下建立和维持。

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