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首页> 外文期刊>Journal of Environmental Science and Health >Response of CYP1A Gene expression in fish liver of catfish (Ariopsis felis) from Gulf of Mexico and their relationship with the genetic variability
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Response of CYP1A Gene expression in fish liver of catfish (Ariopsis felis) from Gulf of Mexico and their relationship with the genetic variability

机译：CYP1A基因表达在墨西哥湾cat鱼鱼肝中的响应及其与遗传变异的关系

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摘要

We determined the hepatic Cytochrome P4501A (CYP1A) mRNA and Ethoxyresorufin-O-deethylase (EROD) activities in the fish, Ariopsis felis, from highly polluted to relatively pristine regions in the southwest Gulf of Mexico and their relationship with the genetic polymorphisms of the same fish. We hypothesized that a high genetic variation reflects interindividual variability in levels of CYP1A mRNA underlying the pathway culminating in EROD induction caused by the environmental contaminants. Catfish from Laguna de Mecoacan exhibited marked induction of CYP1A mRNA and high levels of hepatic EROD activities, whereas fish from Laguna de Celestun showed no induction of CYP1A mRNA and moderately low levels of EROD activities. In contrast, the similarity index considering all samples varied from 0.4 to 0.87, showing a wide range of variation. A dendrogram showed a clear grouping of fish collected from the Laguna de Terminos, Rio Coatzacoalcos and Laguna de Celestun, with discrete subgroups according to region. In contrast, fish from Laguna de Mecoacan were grouped together completely separate from the rest of the fish. Despite the low number of fish from Mecoacan (a high bootstrap support was observed in this group), the results indicated a significant genetic variability in comparison with the other ecosystems included. The differential level of expression of CYP1A and the EROD activity observed among the ecosystems analyzed could be due to the high range of genetic variation, with special emphasis on fish collected in Mecoacan where it is possible to find a subspecies of Ariopsis felis.
机译：我们确定了墨西哥西南海湾鱼类从高污染区到相对原始区的肝脏细胞色素P4501A（CYP1A）mRNA和乙氧基-异色氨酸-O-脱乙基酶（EROD）活性及其与它们的遗传多态性的关系鱼。我们假设一个高的遗传变异反映了CYP1A mRNA水平的个体差异，该CYP1A mRNA的水平最终导致环境污染物引起的EROD诱导。 Laguna de Mecoacan的鱼表现出明显的CYP1A mRNA诱导和高水平的肝EROD活性，而Laguna de Celestun的鱼未表现出CYP1A mRNA的诱导和EROD活性较低。相反，考虑所有样本的相似性指数从0.4到0.87不等，显示出很大的变化范围。树状图显示从拉古纳德特米诺斯，里约Coatzacoalcos和拉古纳德塞莱斯顿收集的鱼的清晰分组，并按地区分为不同的亚组。相比之下，来自拉古纳·德·米科阿坎（Laguna de Mecoacan）的鱼与其他鱼完全分开分类。尽管来自Mecoacan的鱼类数量少（在该组中观察到了很高的自举支持），但结果表明与所包括的其他生态系统相比，其遗传变异性显着。 CYP1A的表达水平和EROD活性的差异在分析的生态系统中可能是由于遗传变异范围广，特别是对在Mecoacan收集的鱼类特别有可能发现了Ariopsis felis的一个亚种。

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    OMAR ZAPATA-PEREZ; LORENZO F. SANCHEZ-TEYER; MARIA T. PEREZ-NUNEZ; ANA L. ARROYO-HERRERA; ADRIANA QUIROZ MORENO; ARNULFO ALBORES-MEDINA;

  • 作者单位

    Departamento de Recursos el Mar, Centro de Investigation y Estudios Avanzados del Instituto Politecnico National, Unidad Merida, Mexico;

    Unidad de Biotecnologia, Centro de Investigation Cientifica de Yucatan, Merida, Mexico;

    Departamento de Recursos el Mar, Centro de Investigation y Estudios Avanzados del Instituto Politecnico National, Unidad Merida, Mexico;

    Departamento de Recursos el Mar, Centro de Investigation y Estudios Avanzados del Instituto Politecnico National, Unidad Merida, Mexico;

    Unidad de Biotecnologia, Centro de Investigation Cientifica de Yucatan, Merida, Mexico;

    Section Externa de Toxicologia. Centro de Investigation y Estudios Avanzados del Instituto Politecnico National, Mexico;

  • 收录信息 美国《科学引文索引》(SCI);美国《工程索引》(EI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
  • 原文格式 PDF
  • 正文语种 eng
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  • 关键词

    biomarkers; gene expression; CYP1A; AFLP; ariopsis felis;

    机译：生物标志物基因表达;CYP1A;AFLP;香气;

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