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首页> 外文期刊>Journal of Environmental Science and Health. A, Toxic/Hazardous Substances & Environmental Engineering >Biodegradation And Detoxication Of Phenol By Using Free And Immobilized Cells Of Acinetobacter Sp. Xa05 And Sphingomonas Sp. Fg03
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Biodegradation And Detoxication Of Phenol By Using Free And Immobilized Cells Of Acinetobacter Sp. Xa05 And Sphingomonas Sp. Fg03

机译：利用不动和固定的不动杆菌属细胞对苯酚进行生物降解和解毒Xa05和Sphingomonas Sp。 Fg03

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摘要

Strain XA05 and FG03 with high biodegradation activity of phenol were isolated from the activated sludge and phenol-contaminated soils in Northwest of China, respectively. DNA sequencing and homologous analysis of 16s rRNA gene identified that XA05 belonged to an Acinetobacter sp. and FG03 was closely related to the Sphingomonas sp. Cells of strain XA05 and FG03 were mixed at the ratio of 1:1, and polyvinyl alcohol (PVA) was used as a gel matrix to immobilize mixed cells by repeated freezing and thawing. Biodegradation was evaluated by determining phenol. Detoxication was evaluated by using Daphnia magna toxicity tests. The removal effciency of phenol and factors affecting phenol degradation were investigated, the stability of the immobilized cells was also reported. Experimental values indicated that both free cells and immobilized cells showed high phenol degradation effciencies, higher than 95% within 35 h with an initial concentration of 800 mg/L phenol, and the immobilized cells showed better performance than that of the suspended-culture cells. These results indicate that immobilized Acinetobncter sp. XA05 and Sphingomonas sp. FG03 possesses a good application potential in the treatment of phenol-containing wastewater.
机译：分别从中国西北地区的活性污泥和苯酚污染的土壤中分离出了具有较高苯酚生物降解活性的菌株XA05和FG03。 DNA测序和16s rRNA基因的同源分析确定XA05属于不动杆菌属。 FG03与Sphingomonas sp。密切相关。 XA05和FG03菌株的细胞以1：1的比例混合，聚乙烯醇（PVA）用作凝胶基质，通过反复冷冻和融化固定混合细胞。通过测定苯酚来评估生物降解。通过使用大型蚤（Daphnia magna）毒性试验评估脱毒。研究了苯酚的去除效率和影响苯酚降解的因素，还报道了固定化细胞的稳定性。实验值表明，游离细胞和固定化细胞均表现出较高的苯酚降解效率，初始浓度为800 mg / L苯酚时，在35 h内降解率高于95％，并且固定化细胞表现出比悬浮培养细胞更好的性能。这些结果表明固定化的不动杆菌。 XA05和鞘氨醇单胞菌sp。 FG03在含酚废水处理中具有良好的应用潜力。

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