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首页> 外文期刊>Journal of Environmental Science and Health. A, Toxic/Hazardous Substances & Environmental Engineering >Growth Inhibition And Biodegradation Of Catecholamines In The Ciliated Protozoan Tetrahymena Pyriformis
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Growth Inhibition And Biodegradation Of Catecholamines In The Ciliated Protozoan Tetrahymena Pyriformis

机译：纤毛虫原虫四膜虫对儿茶酚胺的生长抑制和生物降解

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摘要

A 96-well plate culture methodology for the unicellular eukaryote Tetrahymena pyriformis, strain GL was used for the determination of toxicity and metabolism of catecholamines. Catecholamines exhibited moderate acute toxicity to Tetrahymena cells where dopamine and L-DOPA showed higher toxic potential at EC_(10) (0.39 ppm and 0.63 ppm, respectively) and EC_(20) (1.1 ppm and 1.0 ppm respectively) after 48 h exposure. All tested catecholamines were highly degradable in the PPY-medium due to the oxidizing environment during incubation. Also the catecholamines were naturally synthesized and released by Tetrahymena cells into the culture medium and increasingly accumulated with time where noradrenalin exhibited the highest degree of accumulation. However, the exogenous exposure of catecholamines to the cells caused the depletion of natural noradrenalin synthesis even with the addition of very low physiological concentration (0.12 ppm). Dopamine caused the higher effect on inhibiting noradrenalin synthesis. Treatment with a higher concentration (8.0 ppm) of dopamine in 96-well plates caused strong excitation of the cells and ascertained a new metabolite in vivo while the other representative catecholamines were not responsible for the production of this metabolite. This dopamine metabolite is relatively non-polar as compared to noradrenalin, adrenaline and dopamine and eluting later through the reverse phase C-18 column.
机译：用于单细胞真核生物四膜虫，菌株GL的96孔平板培养方法用于测定儿茶酚胺的毒性和代谢。儿茶酚胺对四膜虫细胞表现出中度急性毒性，暴露48小时后，多巴胺和L-DOPA在EC_（10）（分别为0.39 ppm和0.63 ppm）和EC_（20）（分别为1.1 ppm和1.0 ppm）处显示出更高的毒性潜力。由于孵育过程中的氧化环境，所有测试的儿茶酚胺在PPY培养基中都是高度可降解的。此外，儿茶酚胺是天然合成的，并由四膜虫细胞释放到培养基中，并随着时间的推移而逐渐积累，其中去甲肾上腺素表现出最高的积累程度。然而，儿茶酚胺对细胞的外源暴露导致天然去甲肾上腺素合成的消耗，即使添加了非常低的生理浓度（0.12 ppm）。多巴胺对抑制去甲肾上腺素的合成具有更高的作用。在96孔板中以较高浓度（8.0 ppm）的多巴胺处理可引起细胞强烈刺激，并在体内确定了新的代谢产物，而其他代表性儿茶酚胺则不负责该代谢产物的产生。与去甲肾上腺素，肾上腺素和多巴胺相比，该多巴胺代谢物是相对非极性的，并且随后通过反相C-18柱洗脱。

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