首页> 外国专利> METHOD FOR ESTIMATION OF CYTOTOXICITY OF BURKHOLDERIA PSEUDOMALLEI STRAINS - MELIOIDOSIS PATHOGEN BASED ON THEIR INFLUENCE ON TETRAHYMENA PYRIFORMIS CTR-FORMATION ACTIVITY

METHOD FOR ESTIMATION OF CYTOTOXICITY OF BURKHOLDERIA PSEUDOMALLEI STRAINS - MELIOIDOSIS PATHOGEN BASED ON THEIR INFLUENCE ON TETRAHYMENA PYRIFORMIS CTR-FORMATION ACTIVITY

机译：基于对梨状四膜虫CTR形成活性的影响的拟南芥假单胞菌菌株的细胞毒性评估方法

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FIELD: biotechnology.;SUBSTANCE: method involves cultivation of Tetrahymena pyriformis on LB broth with antibiotics in the amount required for the experiment. Burkholderia pseudomallei is cultivated on LB medium. Burkholderia pseudomallei is combined with Tetrahymena pyriformis at a ratio of 100:1 to obtain a co-culture and incubated at 28°C in the climatic chamber during the period under study, with the count of the number of trophozoites and cysts formed in the Goryaev's chamber after preliminary disinfection with 10% formalin. Differences in the dynamics of Tetrahymena pyriformis cytogenesis in a co-culture with Burkholderia pseudomallei are revealed in comparison with the control. The cytotoxicity of the strains studied is determined by the quantitative index, which is calculated as the ratio of the number of cysts formed to the total number of tetrachimene cells per each time interval studied. In this case, strains with high cytotoxicity cause cysts formation and destruction at an earlier time compared to the strains with reduced virulence.;EFFECT: invention allows to increase the method sensitivity.;3 dwg, 2 tbl, 2 ex

机译：领域：生物技术;研究对象：该方法涉及使用实验所需量的抗生素在LB肉汤上培养梨形四膜虫。假伯克氏菌在LB培养基上培养。在研究期间，拟假种伯克霍尔德氏菌与梨形四膜虫以100：1的比例组合以获得共培养物，并在气候室中于28°C孵育，并计算在戈里亚耶夫氏菌中形成的滋养体和囊肿的数量初步消毒后，用10％福尔马林消毒。与对照相比，揭示了与拟南伯克霍尔德氏菌共培养的梨形四膜虫细胞发生动力学的差异。所研究菌株的细胞毒性由定量指数确定，该定量指数计算为每个所研究时间间隔内形成的囊肿数量与四甲烯细胞总数之比。在这种情况下，与毒性降低的菌株相比，具有高细胞毒性的菌株在更早的时间引起囊肿的形成和破坏。效果：本发明可以提高方法的灵敏度。3 dwg，2 tbl，2 ex

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公开/公告号RU2623175C1

专利类型
公开/公告日2017-06-22

原文格式PDF
申请/专利权人 FEDERALNOE KAZENNOE UCHREZHDENIE ZDRAVOOKHRANENIYA VOLGOGRADSKIJ NAUCHNO-ISSLEDOVATELSKIJ PROTIVOCHUMNYJ INSTITUT FEDERALNOJ SLUZHBY PO NADZORU V SFERE ZASHCHITY PRAV POTREBITELEJ I BLAGOPOLUCHIYA CHELOVEKA;
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申请/专利号RU20160123838
发明设计人 KOROL EKATERINA VASILEVNA;SHUBNIKOVA ELENA VLADIMIROVNA;MERINOVA LYUDMILA KONSTANTINOVNA;MERINOVA OLGA ANATOLEVNA;SENINA TATYANA VASILEVNA;
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申请日2016-06-15
分类号C12Q1/02;C12N1/20;C12N1/10;C12R1/01;
国家 RU
入库时间 2022-08-21 13:23:29

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