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首页> 外文期刊>Journal of Experimental Botany >Fluctuation of oxidative stress indicators in Salix nigra seeds during priming
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Fluctuation of oxidative stress indicators in Salix nigra seeds during priming

机译：灌丛期黑柳种子氧化应激指标的变化

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摘要

Salix nigra seeds subjected to increased humidification show a decrease in normal germination (NG) during early imbibition followed by a recovery in that parameter at increasing imbibition times. Since photo-oxidized seeds contain high levels of reactive oxygen species (ROS), it is possible to infer that the atypical decrease in NG is a consequence of a higher ROS mobilization at early imbibition and the subsequent recovery from an increase in antioxidant activity. In this study, several oxidative stress indicators were evaluated in photo-oxidized seeds subjected to priming. ROS production was studied using electronic spin resonance spectroscopy, spontaneous chemiluminescence (SCL), spectrophotometry (with XTT), and histochemical (with DAB and NBT) and cytochemical (with CeCl3) techniques. Four indicators of molecular damage were monitored: lipid peroxidation, pigment destruction, protein oxidation, and membrane integrity. Antioxidant activity was evaluated by changes in the enzymes SOD, CAT, APX, and POX. The results revealed that the decrease in NG at the beginning of priming occurs by an oxidative burst, as determined by increases in both SCL and superoxide anion radical ( ). Such oxidative burst generates lipid peroxidation, protein oxidation, and a decrease in both pigment content and enzyme activities. With increasing hydration, damages are progressively reversed and NG restored, which coincides with the increased activity of antioxidant defences. It is proposed that these novel observations regarding the occurrence of an oxidative burst are related to the high basal ROS levels and the high membrane content retained in the mature embryo tissues.
机译：受加湿作用的黑柳种子在早期吸水期间显示出正常发芽（NG）下降，然后在吸水时间增加时恢复了该参数。由于光氧化的种子含有高水平的活性氧（ROS），因此可以推断NG的非典型减少是早期吸水时ROS迁移率更高以及随后从抗氧化剂活性增加中恢复的结果。在这项研究中，在进行底涂处理的光氧化种子中评估了几种氧化应激指标。使用电子自旋共振光谱，自发化学发光（SCL），分光光度法（使用XTT），组织化学（使用DAB和NBT）和细胞化学（使用CeCl 3 ）技术研究了ROS的产生。监测了分子损伤的四个指标：脂质过氧化，色素破坏，蛋白质氧化和膜完整性。通过改变酶SOD，CAT，APX和POX来评估抗氧化活性。结果表明，引发时NG的减少是由氧化性爆发引起的，这取决于SCL和超氧阴离子自由基（）的增加。这种氧化性爆发会导致脂质过氧化，蛋白质氧化，并降低色素含量和酶活性。随着水合作用的增加，损伤逐渐被逆转，NG恢复，这与抗氧化剂防御活性的增加相吻合。提出了关于氧化爆发的这些新发现与成熟胚组织中保留的高基础ROS水平和高膜含量有关。

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    《Journal of Experimental Botany》 |2012年第10期|p.3631-3642|共12页
  • 作者

    Gonzalo Roqueiro12 Sara Maldonado3 María del Carmen Ríos4* and Horacio Maroder2*†;

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    1Instituto de Recursos Biológicos, INTA-Castelar, B1712WAA Hurlingham, Argentina 2Departamento de Ciencias Básicas, Universidad Nacional de Luján, 6700 Luján, Argentina 3Departamento de Biodiversidad y Biología Experimental, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, C1428EGA Buenos Aires, Argentina 4Departamento de Química Biológica, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, C1428EGA Buenos Aires, Argentina;

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