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首页> 外文期刊>Journal of Experimental Botany >Maize meiotic mutants with improper or non-homologous synapsis due to problems in pairing or synaptonemal complex formation
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Maize meiotic mutants with improper or non-homologous synapsis due to problems in pairing or synaptonemal complex formation

机译：由于配对或联会复合体形成问题而具有不适当或非同源突触的玉米减数分裂突变体

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During meiotic prophase homologous chromosomes find each other and pair. Then they synapse, as the linear protein core (axial element or lateral element) of each homologous chromosome is joined together by a transverse central element, forming the tripartite synaptonemal complex (SC). Ten uncloned Zea mays mutants in our collection were surveyed by transmission electron microscopy by making silver-stained spreads of SCs to identify mutants with non-homologous synapsis or improper synapsis. To analyse the mutants further, zyp1, the maize orthologue of the Arabidopsis central element component ZYP1 was cloned and an antibody was made against it. Using antibodies against ZYP1 and the lateral element components AFD1 and ASY1, it was found that most mutants form normal SCs but are defective in pairing. The large number of non-homologous synapsis mutants defective in pairing illustrates that synapsis and pairing can be uncoupled. Of the ten mutants studied, only dsy2 undergoes normal homologous chromosome recognition needed for homologous pairing. The dsy2 mutation fails to maintain the SC. ZYP1 elongation is blocked at zygotene, and only dots of ZYP1 are seen at prophase I. Another mutant, mei*N2415 showed incomplete but homologous synapsis and ASY1 and AFD1 have a normal distribution. Although installation of ZYP1 is initiated at zygotene, its progression is slowed down and not completed by pachytene in some cells and ZYP1 is not retained on pachytene chromosomes. The mutants described here are now available through the Maize Genetics Cooperation Stock Center (http://maizecoop.cropsci.uiuc.edu/).
机译：在减数分裂前期，同源染色体彼此配对。然后它们突触，因为每个同源染色体的线性蛋白核心（轴向元件或侧向元件）通过横向中心元件连接在一起，形成三方突触复合物（SC）。通过收集银染的SCs，通过透射电子显微镜调查了我们收集的十个未克隆的玉米突变体，以鉴定具有非同源突触或突触不当的突变体。为了进一步分析突变体，克隆了拟南芥中枢元件ZYP1的玉米直向同源基因zyp1，并制备了针对其的抗体。使用针对ZYP1的抗体以及侧向成分AFD1和ASY1的抗体，发现大多数突变体均形成正常的SC，但配对存在缺陷。配对中大量的非同源突触突变体说明突触和配对可以解偶联。在研究的十个突变体中，只有dsy2经历了同源配对所需的正常同源染色体识别。 dsy2突变无法维持SC。 ZYP1的延伸受合子阻滞，在前期I仅见ZYP1的点。另一个突变体mei * N2415显示不完整，但同源突触，ASY1和AFD1具有正态分布。尽管ZYP1的安装是从合子烯开始的，但其进程却被减缓了，并且在某些细胞中并未被粗线素完成，并且ZYP1并未保留在粗线素染色体上。现在可以通过玉米遗传学合作储备中心（http://maizecoop.cropsci.uiuc.edu/）获得此处描述的突变体。

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