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首页> 外文期刊>Journal of Experimental Botany >Cross activity of orthologous WRKY transcription factors in wheat and Arabidopsis
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Cross activity of orthologous WRKY transcription factors in wheat and Arabidopsis

机译：直系同源WRKY转录因子在小麦和拟南芥中的交叉活性

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摘要

WRKY proteins are transcription factors involved in many plant processes including plant responses to pathogens. Here, the cross activity of TaWRKY78 from the monocot wheat and AtWRKY20 from the dicot Arabidopsis on the cognate promoters of the orthologous PR4-type genes wPR4e and AtHEL of wheat and Arabidopsis, respectively, was investigated. In vitro analysis showed the ability of TaWRKY78 to bind a –17/+80 region of the wPR4e promoter, containing one cis-acting W-box. Moreover, transient expression analysis performed on both TaWRKY78 and AtWRKY20 showed their ability to recognize the cognate cis-acting elements present in the wPR4e and AtHEL promoters, respectively. Finally, this paper provides evidence that both transcription factors are able to cross-regulate the orthologous PR4 genes with an efficiency slightly lower than that exerted on the cognate promoters. The observation that orthologous genes are subjected to similar transcriptional control by orthologous transcription factors demonstrates that the terminal stages of signal transduction pathways leading to defence are conserved and suggests a fundamental role of PR4 genes in plant defence. Moreover, these results corroborate the hypothesis that gene orthology imply similar gene function and that diversification between monocot and dicot has most likely occurred after the specialization of WRKY function.
机译：WRKY蛋白是涉及许多植物过程的转录因子，包括植物对病原体的反应。在这里，研究了单子叶植物小麦的TaWRKY78和双子叶植物拟南芥的AtWRKY20在直系同源PR4型基因wPR4e和AtHEL的同源启动子上的交叉活性。体外分析显示TaWRKY78结合wPR4e启动子的–17 / + 80区域的能力，该区域包含一个顺式作用的W-box。此外，对TaWRKY78和AtWRKY20进行的瞬时表达分析表明，它们能够识别wPR4e和AtHEL启动子中分别存在的同源顺式作用元件。最后，本文提供了两种转录因子均能够以比同源启动子稍低的效率交叉调节直系同源PR4基因的证据。直系同源基因受到直系同源转录因子相似转录控制的观察结果表明，导致防御的信号转导途径的末端阶段是保守的，并暗示了PR4基因在植物防御中的基本作用。此外，这些结果证实了以下假设：基因正射暗示着相似的基因功能，并且在WRKY功能特化后，最有可能发生了单子叶植物和双子叶植物之间的多样化。

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