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首页> 外文期刊>Journal of Experimental Botany >Mining the surface proteome of tomato (Solanum lycopersicum) fruit for proteins associated with cuticle biogenesis
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Mining the surface proteome of tomato (Solanum lycopersicum) fruit for proteins associated with cuticle biogenesis

机译：挖掘番茄（Solanum lycopersicum）果实的表面蛋白质组中与表皮生物发生有关的蛋白质

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The aerial organs of plants are covered by the cuticle, a polyester matrix of cutin and organic solvent-soluble waxes that is contiguous with the polysaccharide cell wall of the epidermis. The cuticle is an important surface barrier between a plant and its environment, providing protection against desiccation, disease, and pests. However, many aspects of the mechanisms of cuticle biosynthesis, assembly, and restructuring are entirely unknown. To identify candidate proteins with a role in cuticle biogenesis, a surface protein extract was obtained from tomato (Solanum lycopersicum) fruits by dipping in an organic solvent and the constituent proteins were identified by several complementary fractionation strategies and two mass spectrometry techniques. Of the ∼200 proteins that were identified, a subset is potentially involved in the transport, deposition, or modification of the cuticle, such as those with predicted lipid-associated protein domains. These include several lipid-transfer proteins, GDSL-motif lipase/hydrolase family proteins, and an MD-2-related lipid recognition domain-containing protein. The epidermal-specific transcript accumulation of several of these candidates was confirmed by laser-capture microdissection and quantitative reverse transcription-PCR (qRT-PCR), together with their expression during various stages of fruit development. This indicated a complex pattern of cuticle deposition, and models for cuticle biogenesis and restructuring are discussed.
机译：植物的气生器官被表皮，角质的聚酯基质和可溶于表皮的多糖细胞壁的有机溶剂可溶性蜡覆盖。角质层是植物与其环境之间的重要表面屏障，可防止干燥，病虫害。然而，表皮生物合成，组装和重组机制的许多方面是完全未知的。为了鉴定在表皮生物发生中起作用的候选蛋白，通过浸入有机溶剂从番茄（Solanum lycopersicum）果实中获得表面蛋白提取物，并通过几种互补的分级分离策略和两种质谱技术鉴定了组成蛋白。在大约200种蛋白质中，有一部分可能与表皮的运输，沉积或修饰有关，例如那些具有预测的脂质相关蛋白质结构域的蛋白质。这些包括几种脂质转移蛋白，GDSL-基序脂肪酶/水解酶家族蛋白和含有MD-2相关脂质识别域的蛋白。通过激光捕获显微切割和定量逆转录PCR（qRT-PCR）以及它们在果实发育各个阶段的表达，证实了其中一些候选物质的表皮特异性转录物积累。这表明表皮沉积的复杂模式，并讨论了表皮生物发生和重构的模型。

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