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首页> 外文期刊>Journal of Experimental Botany >Proximal–distal patterns of transcription factor gene expression during Arabidopsis root development
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Proximal–distal patterns of transcription factor gene expression during Arabidopsis root development

机译：拟南芥根发育过程中转录因子基因表达的近-远模式

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The expression pattern of genes can identify the cells in which the respective proteins are active during development. As a step towards defining the genetic network that controls the development of roots, a high-throughput method of whole-mount in situ hybridization has been developed that does not require expensive equipment and allows the definition of the expression patterns of 137 transcription factor genes in young developing roots. Of the 137 transcription factors, 81.8% were expressed in the root while 18.2% showed no detectable expression. In all three proximal distal zones (meristem, elongation, and differentiation) of the root, 52.6% were expressed whereas 21.2% were expressed in only two zones. Eight percent of the genes were expressed in a single proximal distal zone. Cell-specific gene expression patterns were also detected. This rapid approach identified potential key regulators of cell differentiation and provides important spatial information for the expression patterns of a large number of transcriptional regulators that function during root development.
机译：基因的表达模式可以识别在发育过程中各个蛋白质在其中活跃的细胞。作为定义控制根系发育的遗传网络的一步，已经开发了一种高通量的原位杂交全过程，不需要昂贵的设备，并且可以定义137个转录因子基因的表达模式。年轻的根源。在137个转录因子中，根系表达81.8％，而未检测到表达的有18.2％。在根的所有三个近端远端区域（分生组织，伸长和分化）中，表达52.6％，而仅在两个区域中表达21.2％。百分之八的基因在单个近端远端区域表达。还检测了细胞特异性基因表达模式。这种快速的方法确定了细胞分化的潜在关键调控因子，并为在根发育过程中起作用的大量转录调控因子的表达模式提供了重要的空间信息。

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