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首页> 外文期刊>Journal of Experimental Botany >Proteomic analysis of pathogenesis-related proteins (PRs) induced by compatible and incompatible interactions of pepper mild mottle virus (PMMoV) in Capsicum chinense L3 plants
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Proteomic analysis of pathogenesis-related proteins (PRs) induced by compatible and incompatible interactions of pepper mild mottle virus (PMMoV) in Capsicum chinense L3 plants

机译：辣椒轻斑驳病毒（PMMoV）互作和互不兼容互作诱导辣椒植物L 3 致病相关蛋白（PRs）的蛋白质组学分析

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摘要

Resistance conferred by the L3 gene is active against most of the tobamoviruses, including the Spanish strain (PMMoV-S), a P1,2 pathotype, but not against certain strains of pepper mild mottle virus (PMMoV), termed P1,2,3 pathotype, such as the Italian strain (PMMoV-I). Both viruses are nearly identical at their nucleotide sequence level (98%) and were used to challenge Capsicum chinense PI159236 plants harbouring the L3 gene in order to carry out a comparative proteomic analysis of PR proteins induced in this host in response to infection by either PMMoV-S or PMMoV-I. PMMoV-S induces a hypersensitive reaction (HR) in C. chinense PI159236 plant leaves with the formation of necrotic local lesions and restriction of the virus at the primary infection sites. In this paper, C. chinense PR protein isoforms belonging to the PR-1, β-1,3-glucanases (PR-2), chitinases (PR-3), osmotin-like protein (PR-5), peroxidases (PR-9), germin-like protein (PR-16), and PRp27 (PR-17) have been identified. Three of these PR protein isoforms were specifically induced during PMMoV-S-activation of C. chinense L3 gene-mediated resistance: an acidic β-1,3-glucanase isoform (PR-2) (Mr 44.6; pI 5.1), an osmotin-like protein (PR-5) (Mr 26.8; pI 7.5), and a basic PR-1 protein isoform (Mr 18; pI 9.4–10.0). In addition, evidence is presented for a differential accumulation of C. chinense PR proteins and mRNAs in the compatible (PMMoV-I)–C. chinense and incompatible (PMMoV-S)–C. chinense interactions for proteins belonging to all PR proteins detected. Except for an acidic chitinase (PR-3) (Mr 30.2; pI 5.0), an earlier and higher accumulation of PR proteins and mRNAs was detected in plants associated with HR induction. Furthermore, the accumulation rates of PR proteins and mRNA did not correlate with maximal accumulation levels of viral RNA, thus indicating that PR protein expression may reflect the physiological status of the plant.
机译：L 3 基因赋予的抗药性对大多数烟草花叶病毒具有活性，包括西班牙毒株（PMMoV-S），P 1,2 病态型，但对某些胡椒轻斑驳病毒（PMMoV）毒株，称为P 1,2,3 病态型，例如意大利毒株（PMMoV-1）。两种病毒在核苷酸序列水平上几乎相同（98％），并用于挑战带有L 3 基因的辣椒辣椒PI159236植物，以便对由此诱导的PR蛋白进行蛋白质组学比较分析。感染PMMoV-S或PMMoV-I感染宿主。 PMMoV-S会在C. chinense PI159236植物叶片中引起超敏反应（HR），形成坏死性局部损伤，并限制病毒在主要感染部位的生长。本文中的中华梭菌PR蛋白同工型属于PR-1，β-1,3-葡聚糖酶（PR-2），几丁质酶（PR-3），渗透压样蛋白（PR-5），过氧化物酶（PR -9），胚芽蛋白样蛋白（PR-16）和PRp27（PR-17）。在PMMoV-S激活中华绒毛线虫L 3 基因介导的抗性过程中，特异性诱导了其中的三种PR蛋白亚型：酸性β-1,3-葡聚糖酶亚型（PR-2）（M r 44.6； pI 5.1），渗透压样蛋白（PR-5）（M r 26.8； pI 7.5）和基本PR-1蛋白同工型（M r 18； pI 9.4–10.0）。此外，还提供了在相容的（PMMoV-I）–C中中华绒毛线虫PR蛋白和mRNA差异积累的证据。 chinense和不兼容（PMMoV-S）–C。属于所有检测到的PR蛋白的蛋白质的chinense相互作用。除了酸性几丁质酶（PR-3）（M 3 30.2; pI 5.0），在与HR诱导有关的植物中检测到PR蛋白和mRNA的积累更早，更高。此外，PR蛋白和mRNA的积累速率与病毒RNA的最大积累水平不相关，因此表明PR蛋白的表达可能反映了植物的生理状态。

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