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首页> 外文期刊>Journal of Experimental Botany >Histological characterization of root-knot nematode resistance in cowpea and its relation to reactive oxygen species modulation
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Histological characterization of root-knot nematode resistance in cowpea and its relation to reactive oxygen species modulation

机译：cow豆根结线虫抗性的组织学表征及其与活性氧调制的关系

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Root-knot nematodes (Meloidogyne spp.) are sedentary endoparasites with a broad host range which includes economically important crop species. Cowpea (Vigna unguiculata L. Walp) is an important food and fodder legume grown in many regions where root-knot nematodes are a major problem in production fields. Several sources of resistance to root-knot nematode have been identified in cowpea, including the widely used Rk gene. As part of a study to elucidate the mechanism of Rk-mediated resistance, the histological response to avirulent M. incognita feeding of a resistant cowpea cultivar CB46 was compared with a susceptible near-isogenic line (in CB46 background). Most root-knot nematode resistance mechanisms in host plants that have been examined induced a hypersensitive response (HR). However, there was no typical HR in resistant cowpea roots and nematodes were able to develop normal feeding sites similar to those in susceptible roots up to 9–14 d post inoculation (dpi). From 14–21 dpi giant cell deterioration was observed and the female nematodes showed arrested development and deterioration. Nematodes failed to reach maturity and did not initiate egg laying in resistant roots. These results confirmed that the induction of resistance is relatively late in this system. Typically in pathogen resistance HR is closely associated with an oxidative burst (OB) in infected tissue. The level of reactive oxygen species release in both compatible and incompatible reactions during early and late stages of infection was also quantified. Following a basal OB during early infection in both susceptible and resistant roots, which was also observed in mechanically wounded root tissues, no significant OB was detected up to 14 dpi, a profile consistent with the histological observations of a delayed resistance response. These results will be useful to design gene expression experiments to dissect Rk-mediated resistance at the molecular level.
机译：根结线虫（Meloidogyne spp。）是久坐的内寄生物，宿主范围广泛，包括具有重要经济意义的农作物。 pea豆（Vigna unguiculata L. Walp）是一种重要的食品和饲料豆类，生长在许多根结线虫是生产领域主要问题的地区。在cow豆中已经发现了多种抗根结线虫的来源，包括广泛使用的Rk基因。作为阐明Rk介导的抗性机制的研究的一部分，将抗性cow豆栽培品种CB46对无锯齿的M. incognita喂养的组织学反应与易感近等基因系（在CB46背景中）进行了比较。已检查的寄主植物中大多数根结线虫抗性机制均引起超敏反应（HR）。然而，在抗性cow豆根中没有典型的HR，线虫能够在接种后长达9-14 d（dpi）发育出与易感根相似的正常觅食部位。从14-21 dpi观察到巨大的细胞退化，雌性线虫显示出发育停滞和退化。线虫未能成熟，也没有在抗性根部开始产卵。这些结果证实了在该系统中电阻的诱导相对较晚。通常，在病原体抗性中，HR与感染组织中的氧化爆发（OB）密切相关。在感染的早期和晚期，相容性和不相容性反应中释放的活性氧种类的水平也得到了定量。在易感根和抗性根的早期感染期间进行基础OB（在机械受伤的根组织中也观察到）后，直到14 dpi都未检测到显着的OB，这与延迟抗性反应的组织学观察一致。这些结果将有助于设计基因表达实验，以在分子水平上分析Rk介导的抗性。

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