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首页> 外文期刊>Journal of Experimental Botany >Immunocytochemical localization of Pisum sativum TRXs f and m in non-photosynthetic tissues
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Immunocytochemical localization of Pisum sativum TRXs f and m in non-photosynthetic tissues

机译：非光合组织中Pisum sativum TRXs f和m的免疫细胞化学定位

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Plants are the organisms containing the most complex multigenic family for thioredoxins (TRX). Several types of TRXs are targeted to chloroplasts, which have been classified into four subgroups: m, f, x, and y. Among them, TRXs f and m were the first plastidial TRXs characterized, and their function as redox modulators of enzymes involved in carbon assimilation in the chloroplast has been well-established. Both TRXs, f and m, were named according to their ability to reduce plastidial fructose-1,6-bisphosphatase (FBPase) and malate dehydrogenase (MDH), respectively. Evidence is presented here based on the immunocytochemistry of the localization of f and m-type TRXs from Pisum sativum in non-photosynthetic tissues. Both TRXs showed a different spatial pattern. Whilst PsTRXm was localized to vascular tissues of all the organs analysed (leaves, stems, and roots), PsTRXf was localized to more specific cells next to xylem vessels and vascular cambium. Heterologous complementation analysis of the yeast mutant EMY63, deficient in both yeast TRXs, by the pea plastidial TRXs suggests that PsTRXm, but not PsTRXf, is involved in the mechanism of reactive oxygen species (ROS) detoxification. In agreement with this function, the PsTRXm gene was induced in roots of pea plants in response to hydrogen peroxide.
机译：植物是含有硫氧还蛋白（TRX）最复杂的多基因家族的生物。几种类型的TRX靶向叶绿体，已被分为四个亚组：m，f，x和y。其中，TRX f和m是第一个表征的质体TRX，其作为叶绿体中碳同化酶的氧化还原调节剂的功能已得到公认。 TRXs f和m分别根据其还原质体果糖-1,6-双磷酸酶（FBPase）和苹果酸脱氢酶（MDH）的能力来命名。本文基于非光合组织中来自豌豆（Pisum sativum）的f和m型TRX定位的免疫细胞化学方法提供了证据。两种TRX都显示出不同的空间格局。虽然PsTRXm定位在所有分析器官（叶，茎和根）的血管组织中，但PsTRXf定位在木质部血管和血管形成层附近的更特异的细胞中。豌豆质体TRX对两个酵母TRX中都缺乏的酵母突变体EMY63的异源互补分析表明，PsTRXm而不是PsTRXf参与了活性氧（ROS）的解毒机理。与该功能一致，响应过氧化氢在豌豆植物的根中诱导了PsTRXm基因。

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