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首页> 外文期刊>Journal of Experimental Botany >Modulation of heat shock factors accompanies salicylic acid-mediated potentiation of Hsp70 in tomato seedlings
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Modulation of heat shock factors accompanies salicylic acid-mediated potentiation of Hsp70 in tomato seedlings

机译：水杨酸介导的Hsp70增强番茄幼苗热休克因子的调节。

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摘要

In plants, salicylic acid (SA) is a signalling molecule regulating disease resistance responses such as systemic acquired resistance (SAR) and the hypersensitive response (HR), and has been implicated in both basal and acquired thermotolerance. It has been shown that SA enhances heat-induced Hsp/Hsc70 accumulation in plants. To investigate the mechanism of how SA influences the heat shock response (HSR) in plants, tomato seedlings were treated with SA alone, heat shock, or a combination of both before analyses of hsp70 mRNA, heat shock factor (Hsf)–DNA binding, and gene expression of hsp70, hsfA1, hsfA2, and hsfB1. SA alone led to activation of Hsf–DNA binding, but not induction or transcription of hsp70 mRNA. SA had no significant effect on hsfA2 and hsfB1 gene expression, but potentiated the basal levels of hsfA1. In heat-shocked plants, Hsf–DNA binding was established, and increased hsfA1, hsfA2, and hsfB1 expression was followed by accumulation of Hsp70. SA plus heat shock showed enhanced Hsf–DNA binding, enhanced induction of hsp70 mRNA transcription, and gene expression of hsfA1, hsfA2, and hsfB1, resulting in potentiated levels of Hsp/Hsc70. Since increased hsp70 and hsf gene expression coincide with increased levels of Hsp70 accumulation, it is concluded that SA-mediated potentiation of Hsp70 is due to modulation of these Hsfs by SA. In our efforts to understand the role of Hsp70 in heat-related disease susceptibility, the degree of the complexity of the cross-talk between the pathways in which SA is involved, inter alia, the plant defence response, the HSR and thermotolerance, was further underscored.
机译：在植物中，水杨酸（SA）是调节疾病抵抗力反应（例如系统获得性抵抗力（SAR）和超敏反应（HR））的信号分子，并且与基础和获得性耐热性有关。已经显示出SA增强了植物中热诱导的Hsp / Hsc70积累。为了研究SA如何影响植物的热休克反应（HSR）的机制，在分析hsp70 mRNA，热休克因子（Hsf）–DNA结合之前，单独对SA或热激或两者组合使用SA处理番茄幼苗，和hsp70，hsfA1，hsfA2和hsfB1的基因表达。单独使用SA可以激活Hsf-DNA结合，但不能诱导hsp70 mRNA的转录。 SA对hsfA2和hsfB1基因表达没有显着影响，但增强了hsfA1的基础水平。在热激植物中，建立了Hsf-DNA结合，并增加了hsfA1，hsfA2和hsfB1的表达，随后积累了Hsp70。 SA加热休克显示出增强的Hsf-DNA结合，增强的hsp70 mRNA转录诱导以及hsfA1，hsfA2和hsfB1的基因表达，从而导致Hsp / Hsc70的水平升高。由于增加的hsp70和hsf基因表达与增加的Hsp70积累水平相吻合，因此可以得出结论，SA介导的Hsp70增强是由于SA对这些Hsfs的调节。在我们努力了解Hsp70在与热相关的疾病易感性中的作用时，涉及SA的途径之间串扰的复杂程度（尤其是植物防御反应，HSR和耐热性）更加复杂。强调。

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