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Differential regulation of four members of the ACC synthase gene family in plum

机译：梅中ACC合酶基因家族的四个成员的差异调节

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The regulation of ACC synthase (ACS) genes was studied in early (‘Early Golden’) and late (‘Shiro’) Japanese plum cultivars (Prunus salicina L.) in order to determine the role of this gene family in fruit ripening. Of the four Ps-ACS cDNAs isolated, two (Ps-ACS1 and -3) showed differential expression between the two cultivars. Ps-ACS1 accumulated during fruit ripening of ‘Early Golden’ (‘EG’) and ‘Shiro’ (‘SH’) in ethylene-dependent and -independent manners, respectively. Ps-ACS3a transcripts accumulated throughout fruit development and during ‘EG’ fruit ripening. Ps-ACS3b was detected only during ripening of ‘SH’ fruit. Furthermore, Ps-ACS3a transcript accumulation was negatively regulated by ethylene, whereas Ps-ACS3b was positively induced by the hormone. In both cultivars, the expression of Ps-ACS4 and -5 is under positive and negative feedback control by ethylene, respectively. Genetic analyses of ‘EG’ and ‘SH’ cultivars demonstrated that ‘EG’ is homozygous for Ps-ACS3a whereas ‘SH’ is heterozygous for Ps-ACS3 (a/b). The role of ethylene-overproducer 1-like in delaying fruit ripening by interacting with Ps-ACS proteins was also studied. The effect of the plant hormones, auxin, gibberellin, and cytokinin, in regulating ethylene production by promoting the induction of the different Ps-ACS mRNAs in plum was investigated. A model is presented in which differences in Ps-ACS alleles and gene expression between early and late plums are critical in determining the ripening behaviour of the cultivars.
机译：为了确定该基因家族在果实成熟中的作用，在早期（“早金”）和晚期（“ Shiro”）日本李子品种（Prunus salicina L.）中研究了ACC合酶（ACS）基因的调控。在分离的四个Ps-ACS cDNA中，两个（Ps-ACS1和-3）显示出两个品种之间的差异表达。 Ps-ACS1分别以乙烯依赖性和非乙烯依赖性的方式在“早熟的黄金”（“ EG”）和“ Shiro”（“ SH”）的果实成熟过程中积累。 Ps-ACS3a转录本在整个果实发育过程中以及在'EG'果实成熟期间积累。仅在“ SH”果实成熟期间检测到Ps-ACS3b。此外，Ps-ACS3a转录物的积累受到乙烯的负调控，而Ps-ACS3b被该激素正诱导。在两个品种中，Ps-ACS4和-5的表达分别受到乙烯的正反馈和负反馈控制。对“ EG”和“ SH”品种的遗传分析表明，“ EG”对于Ps-ACS3a是纯合子，而“ SH”对于Ps-ACS3（a / b）是杂合子。还研究了乙烯-过量生产者1样通过与Ps-ACS蛋白相互作用来延迟果实成熟的作用。研究了植物激素，生长素，赤霉素和细胞分裂素通过促进李子中不同Ps-ACS mRNA的诱导来调节乙烯产量的作用。提出了一个模型，其中在早期李子和晚期李子之间，Ps-ACS等位基因和基因表达的差异对于确定品种的成熟行为至关重要。

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    《Journal of Experimental Botany》 |2008年第8期|p.2009-2027|共19页
  • 作者

    I. El-Sharkawy12 W. S. Kim1 S. Jayasankar2* A. M. Svircev1 and D. C. W. Brown3;

  • 作者单位

    1Agriculture and Agri-Food Canada, Southern Crop Protection and Food Research Centre. 4902 Victoria Av. N., PO Box 6000 Vineland Station, ON L0R 2E0, Canada 2University of Guelph, Department of Plant Agriculture, 4890 Victoria Av. N., PO Box 7000 Vineland Station, ON L0R 2E0, Canada 3Agriculture and Agri-Food Canada, Southern Crop Protection and Food Research Centre, 1391 Sandford St, London, ON N5V 4T3, Canada;

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