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A role of brassinosteroids in early fruit development in cucumber

机译：油菜素类固醇在黄瓜早期果实发育中的作用

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摘要

Brassinosteroids (BRs) are essential for many biological processes in plants, however, little is known about their roles in early fruit development. To address this, BR levels were manipulated through the application of exogenous BRs (24-epibrassinolide, EBR) or a BR biosynthesis inhibitor (brassinazole, Brz) and their effects on early fruit development, cell division, and expression of cyclin and cyclin-dependent kinases (CDKs) genes were examined in two cucumber cultivars that differ in parthenocarpic capacity. The application of EBR induced parthenocarpic growth accompanied by active cell division in Jinchun No. 4, a cultivar without parthenocarpic capacity, whereas Brz treatment inhibited fruit set and, subsequently, fruit growth in Jinchun No. 2, a cultivar with natural parthenocarpic capacity, and this inhibitory effect could be rescued by the application of EBR. RT-PCR analysis showed both pollination and EBR induced expression of cell cycle-related genes (CycA, CycB, CycD3;1, CycD3;2, and CDKB) after anthesis. cDNA sequences for CsCycD3;1 and CsCycD3;2 were isolated through PCR amplification. Both CsCycD3;1 and CsCycD3;2 transcripts were up-regulated by EBR treatment and pollination but strongly repressed by Brz treatment. Meanwhile, BR6ox1 and SMT transcripts, two genes involved in BR synthesis, exhibited feedback regulation. These results strongly suggest that BRs play an important role during early fruit development in cucumber.
机译：芸苔素类固醇（BRs）对于植物中许多生物过程都是必不可少的，但是，对于它们在早期果实发育中的作用知之甚少。为了解决这个问题，可通过应用外源BR（24-表油菜素内酯，EBR）或BR生物合成抑制剂（Brassinazole，Brz）来控制BR水平，以及它们对早期水果发育，细胞分裂以及细胞周期蛋白和细胞周期蛋白依赖性表达的影响在两个单性结实能力不同的黄瓜品种中检查了激酶（CDKs）基因。在没有单性结实能力的品种金春4号中，EBR的应用诱导了单性结实的生长并伴随着活跃的细胞分裂，而Brz处理抑制了果实的结实，随后抑制了具有自然单性结实能力的金春2号的果实生长。这种抑制作用可以通过应用EBR得以挽救。 RT-PCR分析显示，授粉后花粉和EBR诱导了细胞周期相关基因（CycA，CycB，CycD3; 1，CycD3; 2和CDKB）的表达。通过PCR扩增分离出CsCycD3； 1和CsCycD3； 2的cDNA序列。 CsCycD3; 1和CsCycD3; 2转录本均通过EBR处理和授粉上调，但被Brz处理强烈抑制。同时，参与BR合成的两个基因BR6ox1和SMT转录物表现出反馈调节。这些结果强烈表明，BRs在黄瓜的早期果实发育过程中起着重要作用。

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  • 来源
    《Journal of Experimental Botany》 |2008年第9期|p.2299-2308|共10页
  • 作者

    Feng Qing Fu1 Wei Hua Mao1 Kai Shi1 Yan Hong Zhou1 Tadao Asami34 and Jing Quan Yu12*;

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    1Department of Horticulture, Huajiachi Campus, Zhejiang University, Kaixuan Road 268, Hangzhou, PR China 310029 2Key Laboratory of Horticultural Plants Growth, Development and Biotechnology, Agricultural Ministry of China, Kaixuan Road 268, Hangzhou, PR China 310029 3RIKEN, Discovery Research Institute, Wako, Saitama 3510198, Japan 4Department of Applied Biological Chemistry, University of Tokyo, Bunkyo Ku, Tokyo 1138657, Japan;

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