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首页> 外文期刊>Journal of Experimental Botany >Response of plasma membrane H+-ATPase to heavy metal stress in Cucumis sativus roots
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Response of plasma membrane H+-ATPase to heavy metal stress in Cucumis sativus roots

机译：黄瓜根质膜H + -ATPase对重金属胁迫的响应

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摘要

The effect of heavy metals on the modification of plasma membrane H+-ATPase (EC 3.6.3.14) activity in cucumber roots was studied. In plants stressed for 2 h with 10 μM or 100 μM Cd, Cu or Ni the hydrolytic as well as the transporting activity of H+-ATPase in the plasma membranes of root cells was decreased. Transcript levels of Cucumis sativus plasma membrane H+-ATPase in roots treated with 10 μM Cd, Cu, or Ni as well as with 100 μM Cu or Ni were similar to the control, indicating that the action of metals did not involve the gene expression level. Only in roots exposed to 100 μM Cd was the level of plasma membrane H+-ATPase mRNA markedly decreased. The inhibition of the plasma membrane proton pump caused by 100 μM Cd, Cu and Ni was partially diminished in the presence of cantharidin, a specific inhibitor of protein phosphatases. Western blot analysis with the antibody against phosphothreonine confirmed that decreased activity of plasma membrane H+-ATPase under heavy metals resulted from dephosphorylation of the enzyme protein. Taken together, these data strongly indicated that alteration of the enzyme under heavy metal stresses was mainly due to the post-translational modification of its proteins in short-term experiments.
机译：研究了重金属对黄瓜根系质膜H + -ATPase（EC 3.6.3.14）活性的影响。在10μM或100μMCd，Cu或Ni胁迫2 h的植物中，H + -ATPase在根细胞质膜中的水解以及转运活性均降低。用10μMCd，Cu或Ni以及100μMCu或Ni处理的根中黄瓜的黄瓜质膜H + -ATPase的转录水平与对照相似，表明金属不涉及基因表达水平。仅在暴露于100μMCd的根中，质膜H + -ATPase mRNA的水平显着降低。在蛋白质蛋白磷酸酶的特异性抑制剂鸟can素的存在下，由100μMCd，Cu和Ni引起的质膜质子泵的抑制作用被部分减弱。磷酸苏氨酸抗体的蛋白质印迹分析证实，重金属下质膜H + -ATPase的活性降低是由于该酶蛋白的去磷酸化所致。综上所述，这些数据强烈表明在重金属胁迫下酶的改变主要是由于短期实验中其蛋白质的翻译后修饰。

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