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首页> 外文期刊>Journal of Experimental Botany >Comparative phylogenetic analysis of small GTP-binding genes of model legume plants and assessment of their roles in root nodules
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Comparative phylogenetic analysis of small GTP-binding genes of model legume plants and assessment of their roles in root nodules

机译：豆科植物模型小GTP结合基因的系统发育比较分析及其在根瘤中的作用

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Small GTP-binding genes play an essential regulatory role in a multitude of cellular processes such as vesicle-mediated intracellular trafficking, signal transduction, cytoskeletal organization, and cell division in plants and animals. Medicago truncatula and Lotus japonicus are important model plants for studying legume-specific biological processes such as nodulation. The publicly available online resources for these plants from websites such as http://www.ncbi.nih.gov, http://www.medicago.org, http://www.tigr.org, and related sites were searched to collect nucleotide sequences that encode GTP-binding protein homologues. A total of 460 small GTPase sequences from several legume species including Medicago and Lotus, Arabidopsis, human, and yeast were phyletically analysed to shed light on the evolution and functional characteristics of legume-specific homologues. One of the main emphases of this study was the elucidation of the possible involvement of some members of small GTPase homologues in the establishment and maintenance of symbiotic associations in root nodules of legumes. A high frequency of vesicle-mediated trafficking in nodules led to the idea of a probable subfunctionalization of some members of this family in legumes. As a result of the analyses, a group of 10 small GTPases that are likely to be mainly expressed in nodules was determined. The sequences determined as a result of this study could be used in more detailed molecular genetic analyses such as creation of RNA inteference silencing mutants for further clarification of the role of GTPases in nodulation. This study will also assist in furthering our understanding of the evolutionary history of small GTPases in legume species.
机译：小GTP结合基因在多种细胞过程中起着至关重要的调节作用，例如囊泡介导的细胞内运输，信号转导，细胞骨架组织以及动植物的细胞分裂。紫花苜蓿和日本莲是研究豆类特定生物过程（例如结瘤）的重要模型植物。搜索了这些植物从http：//www.ncbi.nih.gov、http：//www.medicago.org、http：//www.tigr.org等网站的公开可用资源，以及相关站点，收集编码GTP结合蛋白同源物的核苷酸序列。系统分析了来自包括豆科植物和莲花，拟南芥，人和酵母在内的几种豆科植物物种的总共460条小GTP酶序列，以阐明豆科植物特异性同源物的进化和功能特性。这项研究的主要重点之一是阐明了一些小GTPase同源物可能参与了豆科植物根瘤中共生缔合的建立和维持。囊泡介导的结节贩运的频率很高，导致了这个家庭的一些豆类成员可能亚功能化的想法。作为分析的结果，确定了可能主要在结节中表达的一组10个小的GTP酶。这项研究确定的序列可用于更详细的分子遗传分析，例如创建RNA干扰沉默突变体，以进一步阐明GTPases在结瘤中的作用。这项研究还将有助于加深我们对豆类中小GTP酶进化史的了解。

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    《Journal of Experimental Botany》 |2008年第14期|p.3831-3844|共14页
  • 作者

    Bayram Yuksel* and Abdul R. Memon;

  • 作者单位

    Plant Molecular Biology Laboratory, Genetic Engineering and Biotechnology Institute, Marmara Research Center, TUBITAK, PO Box 21, 41400, Gebze, Kocaeli, Turkey;

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