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首页> 外文期刊>Journal of Experimental Botany >Involvement of EIN3 homologues in basic PR gene expression and flower development in tobacco plants
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Involvement of EIN3 homologues in basic PR gene expression and flower development in tobacco plants

机译：EIN3同源基因参与烟草植物基本PR基因表达和花朵发育的过程

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The TEIL (Tobacco EIN3-Like) gene is a tobacco homologue of arabidopsis Ethylene Insensitive 3 (EIN3), and the gene product binds an 8 bp sequence in the tobacco PR1a promoter in a sequence specific manner. It was found here that accumulation of TEIL transcript was induced by wounding and preceded basic PR gene expression. To study the downstream signalling pathway of TEIL, TEIL was overexpressed under the control of the constitutive 35S promoter in tobacco plants. In 35S::TEIL lines, basic PR genes, which are wound-, jasmonate-, and ethylene-inducible, were expressed constitutively. Next, the conserved 781 bp sequence among tobacco EIN3-like (EIL) protein genes was introduced as an inverted-repeat (IR) into tobacco to suppress expression of these genes. In two independent IRTEIL lines, the TEIL transcript was not found and transcripts of other tobacco EILs, NtEIL3, and NtEIL5, were reduced. In IRTEIL plants, wound-, jasmonate-, and ACC-induced accumulation of basic PR gene transcripts was significantly inhibited. These results indicate that TEIL functions upstream of tobacco basic PR genes in wound signalling via not only ethylene but also jasmonate. In 35S::TEIL plants, the pistil length of the flower was longer with a slight protrusion of the stigma compared with the control. In IRTEIL plants, the length of the stamens was shorter than the control with significant protrusion of the stigma in the flower. These observations indicate the involvement of tobacco EILs in flower development.
机译：TEIL（烟草EIN3-Like）基因是拟南芥乙烯不敏感3（EIN3）的烟草同源物，该基因产物以序列特异性方式结合烟草PR1a启动子中的8 bp序列。在这里发现，TEIL转录物的积累是由伤口诱导的并且在基本PR基因表达之前。为了研究TEIL的下游信号传导途径，在烟草植物中，在组成型35S启动子的控制下，TEIL被过表达。在35S :: TEIL品系中，组成型表达了可被伤口，茉莉酸酯和乙烯诱导的基本PR基因。接下来，将烟草EIN3样（EIL）蛋白基因中保守的781 bp序列作为反向重复（IR）引入烟草中，以抑制这些基因的表达。在两个独立的IRTEIL品系中，未找到TEIL转录本，而其他烟草EILs NtEIL3和NtEIL5的转录本也减少了。在IRTEIL植物中，伤口，茉莉酸酯和ACC诱导的基本PR基因转录本的积累被显着抑制。这些结果表明，TEIL不仅通过乙烯而且通过茉莉酸酯在伤口信号传导中在烟草基本PR基因的上游起作用。与对照相比，在35S :: TEIL植物中，花的雌蕊长度更长，柱头略有突出。在IRTEIL植物中，雄蕊的长度短于花柱头显着突出的对照。这些观察结果表明烟草EIL参与了花的发育。

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