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首页> 外文期刊>Journal of Experimental Botany >Changes in water status and water distribution in maturing lupin seeds studied by MR imaging and NMR spectroscopy
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Changes in water status and water distribution in maturing lupin seeds studied by MR imaging and NMR spectroscopy

机译：羽扇豆成熟种子水分状况和水分布的MR成像和NMR谱研究

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The changes in water distribution in maturing lupin (Lupinus luteus L.) seeds were visualized with magnetic resonance imaging (MRI). MRI data showed local inhomogeneities of water distribution inside the seed. At the late seed-filling stage the most intense signal was detected in the seed coat and the outer parts of cotyledons in the hilum area, but during maturation drying the decline in MR image intensity was faster in the outer part of the seed than in the central part. The changes in water status were characterized by NMR spectroscopy. Analyses of T2 relaxation times revealed a three-component water proton system in maturing lupin seeds. Three populations of protons found during seed maturation, each with a different magnetic environment causing a different relaxation rate, were correlated with three fractions of water (structural, intracellular, and extracellular) that were observed during seed germination. This study provides evidence that lupin seeds have similar states of the different water components with regard to seed moisture content at two distinct physiological stages, seed maturation and germination. The unique feature of maturing lupin seeds is the presence of the high 1H-NMR signal in areas corresponding to the vascular bundles. Tissue localization of dehydrins showed the presence of dehydrin protein in the area of vascular tissue. An anti-dehydrin antibody detected three polypeptides in lupin embryos with molecular masses of 73, 43 and 28 kDa, respectively. The temporal pattern of dehydrin protein accumulation correlates well with seed desiccation.
机译：成熟的羽扇豆（Lupinus luteus L.）种子中水分分布的变化通过磁共振成像（MRI）可视化。 MRI数据显示了种子内部水分分布的局部不均匀性。在种子灌浆后期，在种皮和子叶的子叶外部检测到最强的信号，但在成熟干燥过程中，种子外部的MR图像强度下降快于种子表面。中央部分。通过NMR光谱表征水状态的变化。 T 2 弛豫时间的分析表明，羽扇豆成熟种子具有三组分水质子系统。在种子成熟过程中发现的三个质子种群，每个具有不同的磁性环境，导致不同的弛豫率，与在种子发芽过程中观察到的三部分水（结构，细胞内和细胞外）相关。这项研究提供的证据表明，羽扇豆种子在两个不同的生理阶段（种子成熟和发芽）具有不同水分成分的相似状态，这取决于种子的水分含量。羽扇豆种子成熟的独特特征是在与维管束相对应的区域中存在高 1 H-NMR信号。脱水蛋白的组织定位显示在血管组织区域中存在脱水蛋白。抗脱水蛋白抗体在羽扇豆胚胎中检测到三种多肽，分子量分别为73、43和28 kDa。脱水蛋白积累的时间模式与种子干燥有关。

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