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首页> 外文期刊>Journal of Experimental Botany >Phylogenetic and functional analysis of Arabidopsis RCI2 genes
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Phylogenetic and functional analysis of Arabidopsis RCI2 genes

机译：拟南芥RCI2基因的系统发生和功能分析

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Six new Arabidopsis thaliana genes (AtRCI2C-H) have been identified that show high homology to AtRCI2A and AtRCI2B. Sequence comparisons revealed that AtRCI2-related genes are widely spread among very different organisms, including other plant species, prokaryotes, fungi, and simply organized animals, and are also organized in gene families. Most RCI2 genes show a similar exon–intron organization, which indicates that they have been structurally conserved during evolution, and encode small, highly hydrophobic proteins containing two putative transmembrane domains. Consistently, the majority of AtRCI2 proteins localize in the plasma membrane. RCI2 proteins exhibit an elevated level of sequence similarity and seem to have evolved from a common ancestor. In spite of their high similarity, conserved subcellular localization, and common origin, experimental evidence is presented suggesting that different RCI2 proteins may have distinct functional roles. Thus, as previously demonstrated for AtRCI2A and AtRCI2B, the newly identified AtRCI2 genes (AtRCI2C–H) are differentially regulated in Arabidopsis organs and in response to abiotic stresses and ABA treatment. Furthermore, only the AtRCI2 proteins that do not contain the C-terminal hydrophilic tail (i.e. AtRCI2A–C and AtRCI2H) are able to complement for the loss of the yeast AtRCI2-related gene PMP3. On the basis of these results, different aspects on the evolution and roles of RCI2 genes are discussed.
机译：已经鉴定出六个新的拟南芥基因（AtRCI2C-H），它们与AtRCI2A和AtRCI2B具有高度同源性。序列比较显示，AtRCI2相关基因广泛分布在非常不同的生物中，包括其他植物物种，原核生物，真菌和简单组织的动物，并且也组织在基因家族中。大多数RCI2基因显示出相似的外显子-内含子结构，这表明它们在进化过程中一直是结构保守的，并编码小的，高度疏水的蛋白质，其中包含两个推定的跨膜结构域。一致地，大多数AtRCI2蛋白位于质膜中。 RCI2蛋白显示出较高的序列相似性水平，并且似乎是从共同祖先进化而来的。尽管它们的高度相似性，保守的亚细胞定位和共同的起源，但实验证据表明，不同的RCI2蛋白可能具有不同的功能。因此，如先前针对AtRCI2A和AtRCI2B所证明的，新鉴定的AtRCI2基因（AtRCI2C–H）在拟南芥器官中受到差异调节，并响应非生物胁迫和ABA处理。此外，只有不包含C末端亲水尾的AtRCI2蛋白（即AtRCI2A–C和AtRCI2H）才能够弥补酵母AtRCI2相关基因PMP3的缺失。基于这些结果，讨论了RCI2基因的进化和作用的不同方面。

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    《Journal of Experimental Botany》 |2007年第16期|4333-4346|共14页
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    Joaquín Medina1 María Luisa Ballesteros1 and Julio Salinas12;

  • 作者单位

    Departamento de Biotecnología Instituto Nacional de Investigación y Tecnología Agraría y Alimentaria (INIA) Carretera de la Coruña Km. 7 28040 Madrid Spain;

    Departamento de Biología de Plantas Centro de Investigaciones Biológicas (CIB-CSIC) Ramiro de Maeztu 9 28040 Madrid Spain;

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