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首页> 外文期刊>Journal of Experimental Botany >Senescence- and drought-related changes in peroxidase and superoxide dismutase isoforms in leaves of Ramonda serbica
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Senescence- and drought-related changes in peroxidase and superoxide dismutase isoforms in leaves of Ramonda serbica

机译：雷蒙达（Ramonda serbica）叶片中与衰老和干旱相关的过氧化物酶和超氧化物歧化酶同工型变化

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摘要

Ramonda sp. (Gesneriaceae) is an endemic and relic plant in a very small group of poikilohydric angiosperms that are able to survive in an almost completely dehydrated state. Senescence- and drought-related changes in the activity of peroxidase (POD; EC 1.11.1.7), ascorbate peroxidase (EC 1.11.1.11), and superoxide dismutase (SOD; EC 1.15.1.1) were determined in leaves of different age and relative water content. The results indicate that different POD isoforms were stimulated during senescence and dehydration. Two of the soluble POD isoforms were anionic with pI 4.5, and two were cationic with pI 9.3 and 9.0. The activity of ascorbate peroxidase remained unchanged either by drought or senescence. For the first time, SOD isoforms have now been determined in this resurrection plant. Several SOD isoforms, all of the Mn type, were found to be anionic with pI 4 and a few others had pI from 5 to 6, while one band of FeSOD with a lower molecular weight was neutral. Rehydration brought about a remarkable decrease over the first hour in the activity of all the antioxidant enzymes examined but activity recovered 1 d after rehydration. The results confirmed that dehydration and senescence caused disturbance in the redox homeostasis of Ramonda leaves, while inducing different POD isoforms. A physiological role of peroxidase reaction with hydroxycinnamic acids in conservation and protection of cellular constituents of desiccated Ramonda leaves is suggested.
机译：Ramonda sp。 （Gesneriaceae）是极少数的弱水被子植物中的一种地方性和遗存植物，能够在几乎完全脱水的状态下生存。在不同年龄和相对年龄的叶片中测定了与衰老和干旱相关的过氧化物酶（POD; EC 1.11.1.7），抗坏血酸过氧化物酶（EC 1.11.1.11）和超氧化物歧化酶（SOD; EC 1.15.1.1）活性的变化。含水量。结果表明在衰老和脱水过程中会刺激不同的POD亚型。可溶性POD同工型中有两个是pI 4.5的阴离子型，两个是pI 9.3和9.0的阳离子型。无论是干旱还是衰老，抗坏血酸过氧化物酶的活性均保持不变。现在，这家复活工厂首次确定了SOD同工型。发现所有Mn型的几种SOD亚型均具有pI 4阴离子，另一些具有5I至6的pI，而其中一条较低分子量的FeSOD呈中性。在最初的一小时内，补液导致所有检查的抗氧化酶的活性显着下降，但补液后1 d活性恢复。结果证实，脱水和衰老会引起Ramonda叶片的氧化还原稳态，同时诱导不同的POD亚型。有人提出过氧化酶与羟基肉桂酸反应在干燥的Ramonda叶片的细胞成分的保存和保护中的生理作用。

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    《Journal of Experimental Botany》 |2006年第8期|1759-1768|共10页
  • 作者

    Sonja Veljovic-Jovanovic; Biljana Kukavica; Branka Stevanovic; and Flavia Navari-Izzo;

  • 作者单位

    Center for Multidisciplinary Studies Belgrade University Kneza Višeslava 1a 11030 Belgrade Serbia and Montenegro;

    Faculty of Biology Belgrade University Takovska 11000 Belgrade Serbia and Montenegro;

    Dipartimento di Chimica e Biotecnologie Agrarie Università degli Studi di Pisa Via del Borghetto 80 56124 Pisa Italy;

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