首页> 外国专利> HUMAN MANGANESE SUPEROXIDE DISMUTASE DNA, ITS DEVELOPMENT, RECOVERY OF HUMAN MAGANESE SUPEROXIDE DISMUTASE, HUMAN MANGANESE SUPEROXIDE DISMUTASE ANALOGUE OR HUMAN MANGANESE SUPEROXIDE DISMUTASE MUTANT, USE AND COMPOSITION THEREOF AND TREATMENT METHOD

HUMAN MANGANESE SUPEROXIDE DISMUTASE DNA, ITS DEVELOPMENT, RECOVERY OF HUMAN MAGANESE SUPEROXIDE DISMUTASE, HUMAN MANGANESE SUPEROXIDE DISMUTASE ANALOGUE OR HUMAN MANGANESE SUPEROXIDE DISMUTASE MUTANT, USE AND COMPOSITION THEREOF AND TREATMENT METHOD

机译：人锰超氧化物歧化酶DNA的开发，恢复，人锰超氧化物歧化酶类似物或人锰超氧化物歧化突变体的使用，组成及治疗方法

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摘要

A double-stranded cDNA molecule which includes DNA encoding human manganese superoxide dismutase has been created. The sequence of one strand of a double-stranded DNA molecule which encodes human manganese superoxide dismutase has been discovered. Such molecules may be introduced in prokaryotic, e.g., bacterial, or eucaryotic, e.g., yeast or mammalian, cells and the resulting cells cultured or grown under suitable conditions so as to produce human manganese superoxide dismutase, human manganese superoxide dismutase analogs or human manganese superoxide dismutase mutants which may then be recovered. By this invention, human manganese superoxide dismutase gene fragments from various plasmids may be ligated to yield a complete genomic human manganese superoxide dismutase gene fragment. Human manganese superoxide dismutase, analogs, or mutants may be used to catalyze the reduction of superoxide radicals, reduce reperfusion injury, prolong the survival time of isolated organs, or treat inflammations. The invention also concerns a method of producing enzymatically active human manganese superoxide dismutase compounds in a bacterial cell which contains and is capable of expressing a DNA sequence encoding the superoxide dismutase compound by maintaining the bac terial cell under suitable conditions and in a suitable production medium. The production medium is supplemented with an amount of Mn++ so that the concentration of Mn++ in the medium is greater than about 2 ppm. Genomic manganese superoxide dismutase DNA should also be capable of expression in eucaryotic cells under suitable conditions. This invention also concerns a method of recovering purified enzymatically active manganese superoxide dismutase, analogs or mutants from bacterial cells. It should also be possible to recover manganese SOD from genomic manganese superoxide dismutase DNA expressed in eucaryotic cells using similar methods. The invention further concerns uses of the human manganese superoxide dismutase, human manganese superoxide dismutase analogs or human manganese superoxide dismutase mutants, various new analogs, compositions of the compounds and methods of treating various disorders using these compositions.

机译：已经创建了包括编码人锰超氧化物歧化酶的DNA的双链cDNA分子。已经发现了编码人锰超氧化物歧化酶的双链DNA分子的一条链的序列。可以将这样的分子引入原核的例如细菌的或真核的例如酵母或哺乳动物的细胞中，并在合适的条件下培养或生长所得的细胞，以产生人锰超氧化物歧化酶，人锰超氧化物歧化酶类似物或人锰超氧化物。歧化酶突变体，然后可以将其回收。通过本发明，可以连接来自各种质粒的人锰超氧化物歧化酶基因片段以产生完整的基因组人锰超氧化物歧化酶基因片段。人锰超氧化物歧化酶，类似物或突变体可用于催化超氧化物自由基的还原，减少再灌注损伤，延长离体器官的存活时间或治疗炎症。本发明还涉及在细菌细胞中生产具有酶活性的人锰超氧化物歧化酶化合物的方法，该细菌细胞含有并能够通过将细菌细胞保持在合适的条件下和合适的生产培养基中来表达编码超氧化物歧化酶化合物的DNA序列。向生产培养基中补充一定量的Mn + +，以使培养基中的Mn + +浓度大于约2 ppm。基因组锰超氧化物歧化酶DNA也应该能够在合适的条件下在真核细胞中表达。本发明还涉及从细菌细胞中回收纯化的具有酶活性的锰超氧化物歧化酶，类似物或突变体的方法。还可以使用类似方法从真核细胞中表达的基因组锰超氧化物歧化酶DNA中回收锰SOD。本发明进一步涉及人锰超氧化物歧化酶，人锰超氧化物歧化酶类似物或人锰超氧化物歧化酶突变体，各种新的类似物，化合物的组合物以及使用这些组合物治疗各种疾病的方法的用途。

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公开/公告号JPS6427470A

专利类型
公开/公告日1989-01-30

原文格式PDF
申请/专利权人 BIO-TECHNOL GENERAL CORP;
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申请/专利号JP19880071731
发明设计人 YAKOBU AARU HAATOMAN;YATSUFUA BETSUKU;ABURAHAMU NIMUROTSUDO;
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申请日1988-03-25
分类号C12N15/09;A61K38/00;A61K38/44;A61K38/45;A61P11/00;A61P29/00;C12N1/21;C12N5/10;C12N9/02;C12R1/19;
国家 JP
入库时间 2022-08-22 06:44:43

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