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首页> 外文期刊>Journal of Experimental Botany >Flavin-containing polyamine oxidase is a hydrogen peroxide source in the oxidative response to the protein phosphatase inhibitor cantharidin in Zea mays L.
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Flavin-containing polyamine oxidase is a hydrogen peroxide source in the oxidative response to the protein phosphatase inhibitor cantharidin in Zea mays L.

机译：含黄素的多胺氧化酶是玉米中对蛋白质磷酸酶抑制剂cantharidin的氧化反应中的过氧化氢来源。

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摘要

In this study, the specific contribution of polyamine oxidase (PAO), a hydrogen peroxide (H2O2)-producing enzyme, to the oxidative burst induced in maize mesocotyl by the phosphatase inhibitor cantharidin was examined. For this purpose, a pharmacological approach was applied using, either in vitro or in vivo, two strong inhibitors of maize PAO (MPAO), N-prenylagmatine (G3) and its structural analogue Ro5, as well as diphenyleneiodonium (DPI), an inhibitor of the phagocyte NAD(P)H oxidase. DPI was shown to be a good MPAO inhibitor in vitro. G3, Ro5, and DPI were very effective in inhibiting in vivo the extracellular accumulation of H2O2 that is released by mesocotyl segments upon spermidine supply. G3 and Ro5 did not show any inhibition in vitro of either horseradish peroxidase or barley oxalate oxidase. Moreover, G3 and Ro5 did not inhibit the extracellular accumulation of superoxide radical that is released in vivo upon NADH supply. G3, Ro5, and DPI strongly affected H2O2 production induced in maize mesocotyl by cantharidin. Histochemical localization of H2O2 in cantharidin-treated mesocotyl cross-sections revealed an increase of H2O2-specific staining in the epidermal and subepidermal tissues. The effect was also inhibited by G3 and DPI. Moreover, an increase in MPAO activity was observed in the same tissues upon cantharidin treatment. All these data suggest that G3 and Ro5 behave as powerful and selective inhibitors of MPAO activity either in vitro or in vivo and that MPAO activity contributes to a major part of the cantharidin-induced H2O2 synthesis in the apoplastic milieu of maize mesocotyl.
机译：在这项研究中，多胺氧化酶（PAO），一种过氧化氢（H 2 O 2 ）产生酶，对玉米中胚轴诱导的氧化猝发的特定贡献。检查磷酸酶抑制剂cantharidin。为此，采用了一种药理学方法，在体内或体外使用了两种玉米PAO的强抑制剂（MPAO），N-早稻草碱（G3）及其结构类似物Ro5以及抑制剂二苯并碘鎓（DPI）。吞噬细胞NAD（P）H氧化酶的表达。 DPI被证明是体外良好的MPAO抑制剂。 G3，Ro5和DPI对体内抑制亚精胺供应后中胚轴片段释放的H 2 O 2 的细胞外积累非常有效。 G3和Ro5在体外对辣根过氧化物酶或大麦草酸氧化酶均未显示任何抑制作用。而且，G3和Ro5不抑制NADH供应后体内释放的超氧化物自由基的细胞外积累。 G3，Ro5和DPI强烈影响斑th素在玉米中胚轴中诱导的H 2 O 2 的产生。 H 2 O 2 在邻苯二酚处理的中胚轴截面中的组织化学定位显示H 2 O 2 -特异性染色。该作用也被G3和DPI抑制。此外，在斑th素处理后的相同组织中观察到MPAO活性增加。所有这些数据表明，G3和Ro5在体外或体内均表现为MPAO活性的强力和选择性抑制剂，并且MPAO活性在斑th素诱导的H 2 O 的主要部分中起着重要作用。 2 在玉米中胚轴质外体环境中的合成

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