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首页> 外文期刊>Journal of Experimental Botany >Analysis of the role of COMATOSE and peroxisomal beta-oxidation in the determination of germination potential in Arabidopsis
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Analysis of the role of COMATOSE and peroxisomal beta-oxidation in the determination of germination potential in Arabidopsis

机译：COMATOSE和过氧化物酶体β-氧化在拟南芥发芽势测定中的作用分析

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Comparative physiological analysis of mutant Arabidopsis seeds under defined environmental conditions was used to analyse the relative contributions of components of peroxisomal beta-oxidation in the control of seed germination potential. The COMATOSE (CTS) and KAT2 loci were shown to play essential roles in regulating germination and establishment potentials, whereas LACS6 and LACS7 loci only influenced establishment following germination. The viability and desiccation tolerance of three different mutant alleles of CTS were shown to be intermediate between that of dormant and non-dormant wild-type seeds. Analysis of ttg-1 cts-1 double mutant seeds demonstrated that the cts lesion did not influence after-ripening capacity. These data demonstrate the importance of peroxisomal beta-oxidation in the control of germination potential, but suggest that breakdown of stored lipid is not an important prerequisite for germination. A function is suggested for CTS following after-ripening within pathways related to the progression of germination prior to radicle emergence.
机译：在限定的环境条件下，对突变拟南芥种子进行比较生理分析，以分析过氧化物酶体β-氧化成分在控制种子发芽潜力中的相对贡献。研究显示，COMATOSE（CTS）和KAT2基因座在调节萌发和建立潜力中起着至关重要的作用，而LACS6和LACS7基因座仅影响发芽后的建立。 CTS的三个不同突变等位基因的生存力和干燥耐性被证明介于休眠和非休眠野生型种子之间。对ttg-1 cts-1双突变体种子的分析表明，cts病变不影响成熟后的能力。这些数据证明了过氧化物酶体β-氧化在控制发芽潜力中的重要性，但表明储存的脂质的分解不是发芽的重要前提。在胚根出现之前，与发芽进程有关的途径中的后成熟后，提示了CTS的功能。

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