...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Journal of Experimental Botany >Cloning of an H+-PPase gene from Thellungiella halophila and its heterologous expression to improve tobacco salt tolerance
【24h】

Cloning of an H+-PPase gene from Thellungiella halophila and its heterologous expression to improve tobacco salt tolerance

机译：嗜盐小球菌H + -PPase基因的克隆及其异源表达提高烟草耐盐性

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

An H+-pyrophosphatase (PPase) gene named TsVP involved in basic biochemical and physiological mechanisms was cloned from Thellungiella halophila. The deduced translation product has similar characteristics to H+-PPases from other species, such as Arabidopsis and rice, in terms of bioinformation. The heterologous expression of TsVP in the yeast mutant ena1 suppressed Na+ hypersensitivity and demonstrated the function of TsVP as an H+-PPase. Transgenic tobacco overexpressing TsVP had 60% greater dry weight than wild-type tobacco at 300 mM NaCl and higher viability of mesophyll protoplasts under salt shock stress conditions. TsVP and AVP1, another H+-PPase from Arabidopsis, were heterologously expressed separately in both the yeast mutant ena1 and tobacco. The salt tolerance of TsVP or AVP1 yeast transformants and transgenic tobacco were improved to almost the same level. The TsVP transgenic tobacco lines TL3 and TL5 with the highest H+-PPase hydrolytic activity were studied further. These transgenic tobacco plants accumulated 25% more solutes than wild-type plants without NaCl stress and 20–32% more Na+ under salt stress conditions. Although transgenic tobacco lines TL3 and TL5 accumulated more Na+ in leaf tissues, the malondialdehyde content and cell membrane damage were less than those of the wild type under salt stress conditions. Presumably, compartmentalization of Na+ in vacuoles reduces its toxic effects on plant cells. This result supports the hypothesis that overexpression of H+-PPase causes the accumulation of Na+ in vacuoles instead of in the cytoplasm and avoids the toxicity of excessive Na+ in plant cells.
机译：从嗜盐菌（Thellungiella halophila）克隆了一个名为TsVP的H + 焦磷酸酶（PPase）基因。在生物信息方面，推导的翻译产物具有与其他物种，例如拟南芥和水稻中的H + -PPases相似的特征。 TsVP在酵母突变体ena1中的异源表达抑制了Na + 的超敏反应，并证明了TsVP作为H + -PPase的功能。在300 mM NaCl中，过表达TsVP的转基因烟草的干重比野生型烟草高60％，并且在盐激胁迫条件下，叶肉原生质体的活力更高。 TsVP和AVP1，另一个来自拟南芥的H + -PPase，分别在酵母突变体ena1和烟草中异源表达。 TsVP或AVP1酵母转化子和转基因烟草的耐盐性提高到几乎相同的水平。进一步研究了具有最高H + -PPase水解活性的TsVP转基因烟草株系TL3和TL5。这些转基因烟草植物比没有NaCl胁迫的野生型植物积聚了25％的溶质，在盐胁迫条件下的Na + 增加了20-32％。尽管转基因烟草TL3和TL5在叶片组织中积累了更多的Na + ，但在盐胁迫条件下，丙二醛含量和细胞膜损伤均低于野生型。据推测，Na + 在液泡中的分隔降低了其对植物细胞的毒性作用。该结果支持以下假设：H + -PPase的过度表达导致Na + 在液泡中而不是在细胞质中积累，并避免了过量的Na +的毒性。 在植物细胞中。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号